Pseudomonas aeruginosa PAO1, PA5224 (pepP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5224
Name
pepP
Replicon chromosome
Genomic location 5881679 - 5883013 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253911.1
GI 15600417
Affymetrix PA5224_pepP_at
Entrez 882288
GenBank AAG08609.1
INSDC AAG08609.1
NCBI Locus Tag PA5224
protein_id(GenBank) gb|AAG08609.1|AE004935_6|gnl|PseudoCAP|PA5224
TIGR NTL03PA05225
UniParc UPI00000C5F62
UniProtKB Acc Q9HTW6
UniProtKB ID Q9HTW6_PSEAE
UniRef100 UniRef100_Q9HTW6
UniRef50 UniRef50_Q9HTW6
UniRef90 UniRef90_Q9HTW6

Product

Feature Type CDS
Coding Frame 1
Product Name
aminopeptidase P
Product Name Confidence: Class 2
Synonyms X-Pro aminopeptidase
proline aminopeptidase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -15.03
Kyte-Doolittle Hydrophobicity Value -0.271
Molecular Weight (kDa) 49.7
Isoelectric Point (pI) 5.09

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WZE HYDROLASE 01/17/17 The structure of Pseudomonas aeruginosa aminopeptidase PepP Pseudomonas aeruginosa PAO1 1.783 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 649 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004805 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5224
Search term: pepP
Search term: aminopeptidase P

Human Homologs

Ensembl 110, assembly GRCh38.p14
X-prolyl aminopeptidase 3 [Source:HGNC Symbol;Acc:HGNC:28052]
E-value: 1.9e-74
Percent Identity: 35.6

References

Sequencing and high expression of aminopeptidase P gene from Escherichia coli HB101.
Yoshimoto T, Tone H, Honda T, Osatomi K, Kobayashi R, Tsuru D
J Biochem 1989 Mar;105(3):412-6
PubMed ID: 2659585
Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone.
Nakahigashi K, Miyamoto K, Nishimura K, Inokuchi H
J Bacteriol 1992 Nov;174(22):7352-9
PubMed ID: 1339425