Pseudomonas aeruginosa PAO1, PA5235 (glpT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5235
Name
glpT
Replicon chromosome
Genomic location 5892910 - 5894256 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_253922.1
GI 15600428
Affymetrix PA5235_glpT_at
Entrez 880661
GenBank AAG08620.1
INSDC AAG08620.1
NCBI Locus Tag PA5235
protein_id(GenBank) gb|AAG08620.1|AE004936_3|gnl|PseudoCAP|PA5235
TIGR NTL03PA05236
UniParc UPI00000C5F6C
UniProtKB Acc Q9HTV5
UniProtKB ID Q9HTV5_PSEAE
UniRef100 UniRef100_W0Z344
UniRef50 UniRef50_A6VE09
UniRef90 UniRef90_A6VE09

Product

Feature Type CDS
Coding Frame 1
Product Name
glycerol-3-phosphate transporter
Product Name Confidence: Class 2
Synonyms glycerol-3-phosphate permease
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.02
Kyte-Doolittle Hydrophobicity Value 0.472
Molecular Weight (kDa) 49.3
Isoelectric Point (pI) 9.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 250 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003795 (801 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5235
Search term: glpT

Human Homologs

Ensembl 110, assembly GRCh38.p14
solute carrier family 37 member 4 [Source:HGNC Symbol;Acc:HGNC:4061]
E-value: 5.5e-34
Percent Identity: 28.6
Ensembl 110, assembly GRCh38.p14
solute carrier family 37 member 4 [Source:HGNC Symbol;Acc:HGNC:4061]
E-value: 5.5e-34
Percent Identity: 28.6
Ensembl 110, assembly GRCh38.p14
solute carrier family 37 member 4 [Source:HGNC Symbol;Acc:HGNC:4061]
E-value: 5.5e-34
Percent Identity: 28.6

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Nucleotide sequence and transcriptional startpoint of the glpT gene of Escherichia coli: extensive sequence homology of the glycerol-3-phosphate transport protein with components of the hexose-6-phosphate transport system.
Eiglmeier K, Boos W, Cole ST
Mol Microbiol 1987 Nov;1(3):251-8
PubMed ID: 3329281
The transmembrane topology of the sn-glycerol-3-phosphate permease of Escherichia coli analysed by phoA and lacZ protein fusions.
Gött P, Boos W
Mol Microbiol 1988 Sep;2(5):655-63
PubMed ID: 3141744