Pseudomonas aeruginosa PAO1, PA5245

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5245
Name
Synonym: yhbL
Replicon chromosome
Genomic location 5906626 - 5907294 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253932.1
GI 15600438
Affymetrix PA5245_at
DNASU PaCD00008259
Entrez 880623
GenBank AAG08630.1
INSDC AAG08630.1
NCBI Locus Tag PA5245
protein_id(GenBank) gb|AAG08630.1|AE004937_2|gnl|PseudoCAP|PA5245
TIGR NTL03PA05246
UniParc UPI00000C5F71
UniProtKB Acc Q9HTU9
UniProtKB ID Q9HTU9_PSEAE
UniRef100 UniRef100_W0Z353
UniRef50 UniRef50_A9JRZ9
UniRef90 UniRef90_W0Z353

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.71
Kyte-Doolittle Hydrophobicity Value 0.058
Molecular Weight (kDa) 23.4
Isoelectric Point (pI) 4.72

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 106 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004825 (525 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5245

Human Homologs

Ensembl 110, assembly GRCh38.p14
glutamine amidotransferase class 1 domain containing 3 [Source:HGNC Symbol;Acc:HGNC:1273]
E-value: 5.0e-52
Percent Identity: 49.8

References

Identification of Escherichia coli proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit.
Ueshima R, Fujita N, Ishihama A
Biochem Biophys Res Commun 1992 Apr 30;184(2):634-9
PubMed ID: 1575737
Isolation of cDNA for a novel human protein KNP-I that is homologous to the E. coli SCRP-27A protein from the autoimmune polyglandular disease type I (APECED) region of chromosome 21q22.3.
Nagamine K, Kudoh J, Minoshima S, Kawasaki K, Asakawa S, Ito F, Shimizu N
Biochem Biophys Res Commun 1996 Aug 14;225(2):608-16
PubMed ID: 8753807