Pseudomonas aeruginosa PAO1, PA5291 (betT2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5291
Name
betT2
Synonym: betT2
Replicon chromosome
Genomic location 5955180 - 5957165 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentGlycine betaine-specific importer functions at high but not at low osmolarity
CommentGlycine betaine-specific importer functions at high but not at low osmolarity

Cross-References

RefSeq NP_253978.1
GI 15600484
Affymetrix PA5291_at
Entrez 877729
GenBank AAG08676.1
INSDC AAG08676.1
NCBI Locus Tag PA5291
protein_id(GenBank) gb|AAG08676.1|AE004941_9|gnl|PseudoCAP|PA5291
TIGR NTL03PA05292
UniParc UPI00000C5F8E
UniProtKB Acc Q9HTR3
UniProtKB ID Q9HTR3_PSEAE
UniRef100 UniRef100_W0Z064
UniRef50 UniRef50_A0KYS7
UniRef90 UniRef90_A6VE95

Product

Feature Type CDS
Coding Frame 1
Product Name
BetT2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (73.45 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.51
Kyte-Doolittle Hydrophobicity Value 0.419
Molecular Weight (kDa) 73.5
Isoelectric Point (pI) 6.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 2
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 255 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001742 (829 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5291
Search term: betT2
Search term: BetT2

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular cloning, physical mapping and expression of the bet genes governing the osmoregulatory choline-glycine betaine pathway of Escherichia coli.
Andresen PA, Kaasen I, Styrvold OB, Boulnois G, Strøm AR
J Gen Microbiol 1988 Jun;134(6):1737-46
PubMed ID: 3065456
DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli.
Lamark T, Kaasen I, Eshoo MW, Falkenberg P, McDougall J, Strøm AR
Mol Microbiol 1991 May;5(5):1049-64
PubMed ID: 1956285
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Pseudomonas syringae BetT is a low-affinity choline transporter that is responsible for superior osmoprotection by choline over glycine betaine.
Chen C, Beattie GA
J Bacteriol 2008 Apr;190(8):2717-25
PubMed ID: 18156257