Pseudomonas aeruginosa PAO1, PA5295 (proE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5295
Name
proE
Replicon chromosome
Genomic location 5960852 - 5962528 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentMutation of proE results in wrinkly colony morphology in P. aeruginosa PAO1 (PMID:32582123)
CommentProE negatively regulates the transcriptional expression of pel and psl (PMID:32582123)

Cross-References

RefSeq NP_253982.1
GI 15600488
Affymetrix PA5295_at
Entrez 880557
GenBank AAG08680.1
INSDC AAG08680.1
NCBI Locus Tag PA5295
protein_id(GenBank) gb|AAG08680.1|AE004942_3|gnl|PseudoCAP|PA5295
TIGR NTL03PA05296
UniParc UPI00000C5F91
UniProtKB Acc Q9HTQ9
UniProtKB ID Q9HTQ9_PSEAE
UniRef100 UniRef100_W0Z1E9
UniRef50 UniRef50_A0A0B7DH21
UniRef90 UniRef90_A6VEA0

Product

Feature Type CDS
Coding Frame 1
Product Name
ProE
Product Name Confidence: Class 1
Synonyms phosphodiesterase regulator of EPS
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.83
Kyte-Doolittle Hydrophobicity Value -0.106
Molecular Weight (kDa) 62.3
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 446 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004870 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5295
Search term: proE
Search term: ProE

Human Homologs

References

Regulation of Exopolysaccharide Production by ProE, a Cyclic-Di-GMP Phosphodiesterase in Pseudomonas aeruginosa PAO1.
Feng Q, Ahator SD, Zhou T, Liu Z, Lin Q, Liu Y, Huang J, Zhou J, Zhang LH
Front Microbiol 2020;11:1226
PubMed ID: 32582123