Pseudomonas aeruginosa PAO1, PA5304 (dadA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5304
Name
dadA
Replicon chromosome
Genomic location 5972178 - 5973476 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253991.1
GI 15600497
Affymetrix PA5304_dadA_at
Entrez 878057
GenBank AAG08689.1
INSDC AAG08689.1
NCBI Locus Tag PA5304
protein_id(GenBank) gb|AAG08689.1|AE004943_5|gnl|PseudoCAP|PA5304
TIGR NTL03PA05305
UniParc UPI0000128DEC
UniProtKB Acc Q9HTQ0
UniProtKB ID DADA1_PSEAE
UniRef100 UniRef100_Q9HTQ0
UniRef50 UniRef50_Q9HTQ0
UniRef90 UniRef90_Q9HTQ0

Product

Feature Type CDS
Coding Frame 1
Product Name
D-amino acid dehydrogenase, small subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (47.14 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.09
Kyte-Doolittle Hydrophobicity Value -0.184
Molecular Weight (kDa) 47.1
Isoelectric Point (pI) 7.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 344 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004876 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5304
Search term: dadA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase.
Lobocka M, Hennig J, Wild J, Kłopotowski T
J. Bacteriol. 1994 Mar;176(5):1500-10
PubMed ID: 7906689
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Regulation and characterization of the dadRAX locus for D-amino acid catabolism in Pseudomonas aeruginosa PAO1.
He W, Li C, Lu CD
J Bacteriol 2011 May;193(9):2107-15
PubMed ID: 21378189