Pseudomonas aeruginosa PAO1, PA5323 (argB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5323
Name
argB
Replicon chromosome
Genomic location 5993791 - 5994696 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254010.1
GI 15600516
Affymetrix PA5323_argB_at
DNASU PaCD00008299
Entrez 879620
GenBank AAG08708.1
INSDC AAG08708.1
NCBI Locus Tag PA5323
protein_id(GenBank) gb|AAG08708.1|AE004945_2|gnl|PseudoCAP|PA5323
TIGR NTL03PA05324
UniParc UPI0000165EDF
UniProtKB Acc Q9HTN2
UniProtKB ID ARGB_PSEAE
UniRef100 UniRef100_B7V5L4
UniRef50 UniRef50_Q4K3S0
UniRef90 UniRef90_Q4K3S0

Product

Feature Type CDS
Coding Frame 1
Product Name
acetylglutamate kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.59
Kyte-Doolittle Hydrophobicity Value 0.184
Molecular Weight (kDa) 31.8
Isoelectric Point (pI) 6.81

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2BUF TRANSFERASE 06/12/05 Arginine Feed-Back Inhibitable Acetylglutamate Kinase PSEUDOMONAS AERUGINOSA 2.95 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-Acetyl-L-Glutamate DB04075 DrugBank 5.1.4 Q9HTN2 Acetylglutamate kinase 100.0 301 1.8e-164

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 579 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004894 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5323
Search term: argB

Human Homologs

References

Primary structure, partial purification and regulation of key enzymes of the acetyl cycle of arginine biosynthesis in Bacillus stearothermophilus: dual function of ornithine acetyltransferase.
Sakanyan V, Charlier D, Legrain C, Kochikyan A, Mett I, Piérard A, Glansdorff N
J Gen Microbiol 1993 Mar;139(3):393-402
PubMed ID: 8473852