Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA5331
|
Name |
pyrE
|
Replicon | chromosome |
Genomic location | 6001399 - 6002040 (- strand) |
Transposon Mutants | 1 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 3 transposon mutants in orthologs |
RefSeq | NP_254018.1 |
GI | 15600524 |
Affymetrix | PA5331_pyrE_at |
DNASU | PaCD00008303 |
Entrez | 878227 |
GenBank | AAG08716.1 |
INSDC | AAG08716.1 |
NCBI Locus Tag | PA5331 |
protein_id(GenBank) | gb|AAG08716.1|AE004945_10|gnl|PseudoCAP|PA5331 |
TIGR | NTL03PA05332 |
UniParc | UPI0000132CCD |
UniProtKB Acc | P50587 |
UniProtKB ID | PYRE_PSEAE |
UniRef100 | UniRef100_Q02E31 |
UniRef50 | UniRef50_O94331 |
UniRef90 | UniRef90_Q02E31 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
orotate phosphoribosyltransferase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -4.31 |
Kyte-Doolittle Hydrophobicity Value | 0.043 |
Molecular Weight (kDa) | 23.3 |
Isoelectric Point (pI) | 5.12 |
Individual Mappings | |
Additional evidence for subcellular localization |
Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.
Drug Name | Source Accession | Source DB | Version | Target Accession | Target Description | Percent Identity | Alignment Length | E-Value |
Orotic Acid | DB02262 | DrugBank | 5.1.4 | P08870 | Orotate phosphoribosyltransferase | 69.0 | 213 | 3.5e-82 |
5-O-phosphono-alpha-D-ribofuranosyl diphosphate | DB01632 | DrugBank | 5.1.4 | P08870 | Orotate phosphoribosyltransferase | 69.0 | 213 | 3.5e-82 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 357 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG004900 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA5331
Search term: pyrE
Search term: orotate phosphoribosyltransferase
|
Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.
Poulsen P, Jensen KF, Valentin-Hansen P, Carlsson P, Lundberg LG
Eur J Biochem 1983 Sep 15;135(2):223-9
PubMed ID: 6349999
|
A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase.
Henriksen A, Aghajari N, Jensen KF, Gajhede M
Biochemistry 1996 Mar 26;35(12):3803-9
PubMed ID: 8620002
|
Accumulation of Pyrimidine Intermediate Orotate Decreases Virulence Factor Production in Pseudomonas aeruginosa.
Niazy A, Hughes LE
Curr Microbiol 2015 Aug;71(2):229-34
PubMed ID: 25917504
|