Pseudomonas aeruginosa PAO1, PA5331 (pyrE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5331
Name
pyrE
Replicon chromosome
Genomic location 6001399 - 6002040 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_254018.1
GI 15600524
Affymetrix PA5331_pyrE_at
DNASU PaCD00008303
Entrez 878227
GenBank AAG08716.1
INSDC AAG08716.1
NCBI Locus Tag PA5331
protein_id(GenBank) gb|AAG08716.1|AE004945_10|gnl|PseudoCAP|PA5331
TIGR NTL03PA05332
UniParc UPI0000132CCD
UniProtKB Acc P50587
UniProtKB ID PYRE_PSEAE
UniRef100 UniRef100_Q02E31
UniRef50 UniRef50_O94331
UniRef90 UniRef90_Q02E31

Product

Feature Type CDS
Coding Frame 1
Product Name
orotate phosphoribosyltransferase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.31
Kyte-Doolittle Hydrophobicity Value 0.043
Molecular Weight (kDa) 23.3
Isoelectric Point (pI) 5.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Orotic Acid DB02262 DrugBank 5.1.4 P08870 Orotate phosphoribosyltransferase 69.0 213 3.5e-82
5-O-phosphono-alpha-D-ribofuranosyl diphosphate DB01632 DrugBank 5.1.4 P08870 Orotate phosphoribosyltransferase 69.0 213 3.5e-82

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 357 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004900 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5331
Search term: pyrE

Human Homologs

References

Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region.
Poulsen P, Jensen KF, Valentin-Hansen P, Carlsson P, Lundberg LG
Eur J Biochem 1983 Sep 15;135(2):223-9
PubMed ID: 6349999
A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase.
Henriksen A, Aghajari N, Jensen KF, Gajhede M
Biochemistry 1996 Mar 26;35(12):3803-9
PubMed ID: 8620002
Accumulation of Pyrimidine Intermediate Orotate Decreases Virulence Factor Production in Pseudomonas aeruginosa.
Niazy A, Hughes LE
Curr Microbiol 2015 Aug;71(2):229-34
PubMed ID: 25917504