Pseudomonas aeruginosa PAO1, PA5332 (crc)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5332
Name
crc
Replicon chromosome
Genomic location 6002121 - 6002900 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254019.1
GI 15600525
Affymetrix PA5332_crc_at
Entrez 878037
GenBank AAG08717.1
INSDC AAG08717.1
NCBI Locus Tag PA5332
protein_id(GenBank) gb|AAG08717.1|AE004946_1|gnl|PseudoCAP|PA5332
TIGR NTL03PA05333
UniParc UPI00000D4294
UniProtKB Acc G3XDB0
UniProtKB ID G3XDB0_PSEAE
UniRef100 UniRef100_W0Z3D9
UniRef50 UniRef50_Q49P76
UniRef90 UniRef90_A6VED9

Product

Feature Type CDS
Coding Frame 1
Product Name
catabolite repression control protein
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.40
Kyte-Doolittle Hydrophobicity Value -0.431
Molecular Weight (kDa) 29.8
Isoelectric Point (pI) 5.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4JG3 UNKNOWN FUNCTION 02/28/13 Crystal structure of catabolite repression control protein (crc) from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 100.0
6O1L RNA BINDING PROTEIN/RNA/HYDROLASE 02/20/19 Architectural principles for Hfq/Crc-mediated regulation of gene expression Hfq-Crc-amiE 2:3:2 complex Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.37 ELECTRON MICROSCOPY 100.0
4F1R TRANSCRIPTION REGULATOR 05/07/12 Structure analysis of the global metabolic regulator Crc from Pseudomonas aeruginos Pseudomonas aeruginosa 2.201 X-RAY DIFFRACTION 100.0
8BVJ RNA BINDING PROTEIN 12/04/22 Hfq-Crc-estA translation repression complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 4.5 ELECTRON MICROSCOPY 100.0
6O1K RNA BINDING PROTEIN/RNA/Hydrolase 02/20/19 Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:2:2 complex (core complex) Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.13 ELECTRON MICROSCOPY 100.0
6O1M RNA BINDING PROTEIN/RNA/HYDROLASE 02/20/19 Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:4:2 complex Pseudomonas aeruginosa; Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.15 ELECTRON MICROSCOPY 100.0
8BVM RNA BINDING PROTEIN 12/04/22 Cryo-EM structure of Hfq-Crc-rbsB translation repression complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.8 ELECTRON MICROSCOPY 100.0
8BVH RNA BINDING PROTEIN 12/03/22 Cryo-EM structure of the Hfq-Crc-amiE translation repression assembly. Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.6 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 492 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004901 (538 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5332
Search term: crc

Human Homologs

References

The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product.
MacGregor CH, Arora SK, Hager PW, Dail MB, Phibbs PV Jr
J Bacteriol 1996 Oct;178(19):5627-35
PubMed ID: 8824606