Pseudomonas aeruginosa PAO1, PA5337 (rpoZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5337
Name
rpoZ
Replicon chromosome
Genomic location 6005899 - 6006165 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_254024.1
GI 15600530
Affymetrix PA5337_rpoZ_at
DNASU PaCD00008307
Entrez 878048
GenBank AAG08722.1
INSDC AAG08722.1
NCBI Locus Tag PA5337
protein_id(GenBank) gb|AAG08722.1|AE004946_6|gnl|PseudoCAP|PA5337
TIGR NTL03PA05338
UniParc UPI0000134801
UniProtKB Acc Q9HTM1
UniProtKB ID RPOZ_PSEAE
UniRef100 UniRef100_A6VEE4
UniRef50 UniRef50_Q5E8P6
UniRef90 UniRef90_A6VEE4

Product

Feature Type CDS
Coding Frame 1
Product Name
RNA polymerase omega subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.03
Kyte-Doolittle Hydrophobicity Value -0.309
Molecular Weight (kDa) 9.8
Isoelectric Point (pI) 3.95

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7VF9 TRANSCRIPTION 09/10/21 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes Pseudomonas aeruginosa PAO1 4.04 ELECTRON MICROSCOPY 100.0
7XYB TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loaded complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.7 ELECTRON MICROSCOPY 100.0
7XL4 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe) Pseudomonas aeruginosa PAO1 3.86 ELECTRON MICROSCOPY 100.0
7F0R TRANSCRIPTION/DNA 06/06/21 Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 5.8 ELECTRON MICROSCOPY 100.0
7XYA TRANSCRIPTION 06/01/22 The cryo-EM structure of an AlpA-loading complex Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 3.3 ELECTRON MICROSCOPY 100.0
7XL3 TRANSCRIPTION 04/21/22 Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe) Pseudomonas aeruginosa PAO1 3.13 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 245 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004906 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5337
Search term: rpoZ

Human Homologs

References

The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity.
Persson BC, Jäger G, Gustafsson C
Nucleic Acids Res 1997 Oct 15;25(20):4093-7
PubMed ID: 9321663