Pseudomonas aeruginosa PAO1, PA5350 (rubA2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5350
Name
rubA2
Replicon chromosome
Genomic location 6018830 - 6018997 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254037.1
GI 15600543
Affymetrix PA5350_r_at
DNASU PaCD00008316
Entrez 879561
GenBank AAG08735.1
INSDC AAG08735.1
NCBI Locus Tag PA5350
protein_id(GenBank) gb|AAG08735.1|AE004947_5|gnl|PseudoCAP|PA5350
TIGR NTL03PA05351
UniParc UPI00000C5FB9
UniProtKB Acc Q9HTK8
UniProtKB ID RUBR2_PSEAE
UniRef100 UniRef100_Q9HTK8
UniRef50 UniRef50_Q9HTK8
UniRef90 UniRef90_Q9HTK8

Product

Feature Type CDS
Coding Frame 1
Product Name
Rubredoxin 2
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -7.14
Kyte-Doolittle Hydrophobicity Value 0.104
Molecular Weight (kDa) 6.2
Isoelectric Point (pI) 3.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2V3B OXIDOREDUCTASE 06/14/07 Crystal structure of the electron transfer complex rubredoxin - rubredoxin reductase from Pseudomonas aeruginosa. PSEUDOMONAS AERUGINOSA 2.45 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 304 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004918 (839 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5350
Search term: rubA2
Search term: Rubredoxin 2

Human Homologs

References

Signal pathways involved in activation of p70S6K and phosphorylation of 4E-BP1 following exposure of multiple myeloma tumor cells to interleukin-6.
Shi Y, Hsu JH, Hu L, Gera J, Lichtenstein A
J Biol Chem 2002 May 3;277(18):15712-20
PubMed ID: 11872747
Rubredoxin from Clostridium thermosaccharolyticum. Amino acid sequence, mass-spectrometric and preliminary crystallographic data.
Meyer J, Gagnon J, Sieker LC, Van Dorsselaer A, Moulis JM
Biochem J 1990 Nov 1;271(3):839-41
PubMed ID: 2244884
Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1.
Haspel G, Ehrt S, Hillen W
Microbiology (Reading) 1995 Jun;141 ( Pt 6):1425-1432
PubMed ID: 7670642