Pseudomonas aeruginosa PAO1, PA5369 (pstS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5369
Name
pstS
Replicon chromosome
Genomic location 6038212 - 6039183 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254056.1
GI 15600562
Affymetrix PA5369_at
DNASU PaCD00008324
Entrez 880528
GenBank AAG08754.1
INSDC AAG08754.1
NCBI Locus Tag PA5369
protein_id(GenBank) gb|AAG08754.1|AE004949_1|gnl|PseudoCAP|PA5369
TIGR NTL03PA05370
UniParc UPI00000D4291
UniProtKB Acc G3XDA8
UniProtKB ID PSTS_PSEAE
UniRef100 UniRef100_Q02DZ3
UniRef50 UniRef50_Q02DZ3
UniRef90 UniRef90_Q02DZ3

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphate ABC transporter, periplasmic phosphate-binding protein, PstS
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 7.17
Kyte-Doolittle Hydrophobicity Value -0.117
Molecular Weight (kDa) 34.5
Isoelectric Point (pI) 9.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4PQJ TRANSPORT PROTEIN 03/03/14 Crystal structure of a phosphate binding protein Pseudomonas aeruginosa 1.86 X-RAY DIFFRACTION 100.0
4OMB TRANSPORT PROTEIN 01/27/14 Phosphate binding protein Pseudomonas aeruginosa 1.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 328 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004934 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5369
Search term: pstS

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway.
Nakada Y, Itoh Y
Microbiology (Reading) 2003 Mar;149(Pt 3):707-714
PubMed ID: 12634339
Phosphate transport in Pseudomonas aeruginosa. Involvement of a periplasmic phosphate-binding protein.
Poole K, Hancock RE
Eur J Biochem 1984 Nov 2;144(3):607-12
PubMed ID: 6436026
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994