Pseudomonas aeruginosa PAO1, PA5399 (dgcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5399
Name
dgcB
Synonym: dgcB
Replicon chromosome
Genomic location 6078044 - 6080005 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
Comment Strains with transposon insertions in dgcB are unable to grow on choline, glycine betaine, or dimethylglcycine but are able to utilize sarcosine. NMR analysis shows buildup of labeled dimethylglycine when dgcB mutants are given labeled choline. Predicted to function with DgcA (PA5398).

Cross-References

RefSeq NP_254086.1
GI 15600592
Affymetrix PA5399_at
Entrez 882141
GenBank AAG08784.1
INSDC AAG08784.1
NCBI Locus Tag PA5399
protein_id(GenBank) gb|AAG08784.1|AE004952_6|gnl|PseudoCAP|PA5399
TIGR NTL03PA05400
UniParc UPI00000C5FE4
UniProtKB Acc Q9HTG5
UniProtKB ID Q9HTG5_PSEAE
UniRef100 UniRef100_Q9HTG5
UniRef50 UniRef50_Q9HTG5
UniRef90 UniRef90_Q9HTG5

Product

Feature Type CDS
Coding Frame 1
Product Name
DgcB, Dimethylglycine catabolism
Product Name Confidence: Class 1
Synonyms DgcB, Dimethylglycine catabolism
Evidence for Translation
Charge (pH 7) 11.93
Kyte-Doolittle Hydrophobicity Value 0.060
Molecular Weight (kDa) 70.5
Isoelectric Point (pI) 8.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 306 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004956 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5399
Search term: dgcB

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379