Pseudomonas aeruginosa PAO1, PA5426 (purE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5426
Name
purE
Replicon chromosome
Genomic location 6106272 - 6106763 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254113.1
GI 15600619
Affymetrix PA5426_purE_at
DNASU PaCD00008343
Entrez 877624
GenBank AAG08811.1
INSDC AAG08811.1
NCBI Locus Tag PA5426
protein_id(GenBank) gb|AAG08811.1|AE004955_5|gnl|PseudoCAP|PA5426
TIGR NTL03PA05427
UniParc UPI0000132A7D
UniProtKB Acc P72157
UniProtKB ID PURE_PSEAE
UniRef100 UniRef100_P72157
UniRef50 UniRef50_Q9KVT7
UniRef90 UniRef90_P72157

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoribosylaminoimidazole carboxylase, catalytic subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.60
Kyte-Doolittle Hydrophobicity Value 0.227
Molecular Weight (kDa) 16.9
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by osmotic shock.(PMID:19333994)

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 627 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004979 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5426
Search term: purE

Human Homologs

Ensembl 94, assembly GRCh38.p12
phosphoribosylaminoimidazole carboxylase and phosphoribosylaminoimidazolesuccinocarboxamide synthase [Source:HGNC Symbol;Acc:HGNC:8587]
E-value: 7.5e-06
Percent Identity: 32.0

References

Large-scale isolation of candidate virulence genes of Pseudomonas aeruginosa by in vivo selection.
Wang J, Mushegian A, Lory S, Jin S
Proc. Natl. Acad. Sci. U.S.A. 1996 Sep;93(19):10434-9
PubMed ID: 8816818
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142