Pseudomonas aeruginosa PAO1, PA5429 (aspA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5429
Name
aspA
Replicon chromosome
Genomic location 6109260 - 6110684 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254116.1
GI 15600622
Affymetrix PA5429_aspA_at
Entrez 878603
GenBank AAG08814.1
INSDC AAG08814.1
NCBI Locus Tag PA5429
protein_id(GenBank) gb|AAG08814.1|AE004955_8|gnl|PseudoCAP|PA5429
TIGR NTL03PA05430
UniParc UPI00000C5FFE
UniProtKB Acc Q9HTD7
UniProtKB ID ASPA_PSEAE
UniRef100 UniRef100_Q9HTD7
UniRef50 UniRef50_P07346
UniRef90 UniRef90_Q9HTD7

Product

Feature Type CDS
Coding Frame 1
Product Name
aspartate ammonia-lyase
Product Name Confidence: Class 2
Synonyms aspartase
Evidence for Translation
Detected using LC-MS/MS (51.07 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -8.46
Kyte-Doolittle Hydrophobicity Value 0.054
Molecular Weight (kDa) 51.1
Isoelectric Point (pI) 5.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 611 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004982 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5429
Search term: aspA

Human Homologs

Ensembl 94, assembly GRCh38.p12
fumarate hydratase [Source:HGNC Symbol;Acc:HGNC:3700]
E-value: 2.3e-95
Percent Identity: 40.9

References

Cloning and nucleotide sequence of the aspartase gene of Pseudomonas fluorescens.
Takagi JS, Tokushige M, Shimura Y
J. Biochem. 1986 Sep;100(3):697-705
PubMed ID: 3096982
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344