Pseudomonas aeruginosa PAO1, PA5441

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5441
Name
Replicon chromosome
Genomic location 6125796 - 6127997 (- strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_254128.1
GI 15600634
Affymetrix PA5441_at
Entrez 877847
GenBank AAG08826.1
INSDC AAG08826.1
NCBI Locus Tag PA5441
protein_id(GenBank) gb|AAG08826.1|AE004957_1|gnl|PseudoCAP|PA5441
TIGR NTL03PA05442
UniParc UPI00000C600A
UniProtKB Acc Q9HTC5
UniProtKB ID Q9HTC5_PSEAE
UniRef100 UniRef100_Q9HTC5
UniRef50 UniRef50_Q9HTC5
UniRef90 UniRef90_Q9HTC5

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (80.04 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.23
Kyte-Doolittle Hydrophobicity Value -0.328
Molecular Weight (kDa) 80.0
Isoelectric Point (pI) 4.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database Victors : 4205
Category
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 14 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004993 (372 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5441

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344