Pseudomonas aeruginosa PAO1, PA5485 (ampDh2)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5485
Name
ampDh2
Replicon chromosome
Genomic location 6176516 - 6177295 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254172.1
GI 15600678
Affymetrix PA5485_at
DNASU PaCD00008362
Entrez 880851
GenBank AAG08870.1
INSDC AAG08870.1
NCBI Locus Tag PA5485
protein_id(GenBank) gb|AAG08870.1|AE004961_5|gnl|PseudoCAP|PA5485
TIGR NTL03PA05486
UniParc UPI00000C6030
UniProtKB Acc Q9HT86
UniProtKB ID Q9HT86_PSEAE
UniRef100 UniRef100_W0Z228
UniRef50 UniRef50_Q4KKJ3
UniRef90 UniRef90_W0Z228

Product

Feature Type CDS
Coding Frame 1
Product Name
AmpDh2
Product Name Confidence: Class 1
Synonyms AmpDh2 (changed from conserved hypothetical protein)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.89
Kyte-Doolittle Hydrophobicity Value -0.296
Molecular Weight (kDa) 28.9
Isoelectric Point (pI) 7.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4BJ4 HYDROLASE 04/16/13 Structure of Pseudomonas aeruginosa amidase Ampdh2 PSEUDOMONAS AERUGINOSA PAO1 1.722 X-RAY DIFFRACTION 100.0
4BOL HYDROLASE 05/21/13 Crystal structure of AmpDh2 from Pseudomonas aeruginosa in complex with pentapeptide PSEUDOMONAS AERUGINOSA PAO1 1.7 X-RAY DIFFRACTION 99.6
4BPA HYDROLASE 05/23/13 Crystal structure of AmpDh2 from Pseudomonas aeruginosa in complex with NAG-NAM-NAG-NAM tetrasaccharide PSEUDOMONAS AERUGINOSA PAO1 2.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 249 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005025 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5485
Search term: ampDh2
Search term: AmpDh2

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Stepwise upregulation of the Pseudomonas aeruginosa chromosomal cephalosporinase conferring high-level beta-lactam resistance involves three AmpD homologues.
Juan C, Moyá B, Pérez JL, Oliver A
Antimicrob Agents Chemother 2006 May;50(5):1780-7
PubMed ID: 16641450