Pseudomonas aeruginosa PAO1, PA5489 (dsbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5489
Name
dsbA
Replicon chromosome
Genomic location 6180942 - 6181577 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254176.1
GI 15600682
Affymetrix PA5489_dsbA_at
DNASU PaCD00008364
Entrez 877731
GenBank AAG08874.1
INSDC AAG08874.1
NCBI Locus Tag PA5489
protein_id(GenBank) gb|AAG08874.1|AE004961_9|gnl|PseudoCAP|PA5489
TIGR NTL03PA05490
UniParc UPI00001298C0
UniProtKB Acc P0C2B2
UniProtKB ID DSBA_PSEAE
UniRef100 UniRef100_Q02DM0
UniRef50 UniRef50_O52376
UniRef90 UniRef90_Q02DM0

Product

Feature Type CDS
Coding Frame 1
Product Name
thiol:disulfide interchange protein DsbA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.38
Kyte-Doolittle Hydrophobicity Value -0.064
Molecular Weight (kDa) 23.4
Isoelectric Point (pI) 6.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZL8 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal II PSEUDOMONAS AERUGINOSA 1.395 X-RAY DIFFRACTION 98.4
4ZL7 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal I PSEUDOMONAS AERUGINOSA 1.922 X-RAY DIFFRACTION 98.4
3H93 TRANSCRIPTION REGULATOR 04/29/09 Crystal Structure of Pseudomonas aeruginosa DsbA PSEUDOMONAS AERUGINOSA PAO1 1.501 X-RAY DIFFRACTION 99.0
4ZL9 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal III PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 98.4
5DCH OXIDOREDUCTASE 08/24/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I in complex with MIPS-0000851 (3-[(2-METHYLBENZYL)SULFANYL]-4H-1,2,4-TRIAZOL-4-AMINE) PSEUDOMONAS AERUGINOSA 1.447 X-RAY DIFFRACTION 98.4
2MBT OXIDOREDUCTASE 08/03/13 NMR study of PaDsbA PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0
5TLQ OXIDOREDUCTASE 10/11/16 Model structure of the oxidized PaDsbA1 and 3-[(2-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-4-amine complex PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 149 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005029 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5489
Search term: dsbA

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and expression of the gene for a protein disulfide oxidoreductase from Azotobacter vinelandii: complementation of an Escherichia coli dsbA mutant strain.
Ng TC, Kwik JF, Maier RJ
Gene 1997 Mar;188(1):109-13
PubMed ID: 9099867
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994