Pseudomonas aeruginosa PAO1, PA5489 (dsbA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5489
Name
dsbA
Replicon chromosome
Genomic location 6180942 - 6181577 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254176.1
GI 15600682
Affymetrix PA5489_dsbA_at
DNASU PaCD00008364
Entrez 877731
GenBank AAG08874.1
INSDC AAG08874.1
NCBI Locus Tag PA5489
protein_id(GenBank) gb|AAG08874.1|AE004961_9|gnl|PseudoCAP|PA5489
TIGR NTL03PA05490
UniParc UPI00001298C0
UniProtKB Acc P0C2B2
UniProtKB ID DSBA_PSEAE
UniRef100 UniRef100_Q02DM0
UniRef50 UniRef50_O52376
UniRef90 UniRef90_Q02DM0

Product

Feature Type CDS
Coding Frame 1
Product Name
thiol:disulfide interchange protein DsbA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.38
Kyte-Doolittle Hydrophobicity Value -0.064
Molecular Weight (kDa) 23.4
Isoelectric Point (pI) 6.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZL7 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal I PSEUDOMONAS AERUGINOSA 1.922 X-RAY DIFFRACTION 98.4
3H93 TRANSCRIPTION REGULATOR 04/29/09 Crystal Structure of Pseudomonas aeruginosa DsbA PSEUDOMONAS AERUGINOSA PAO1 1.501 X-RAY DIFFRACTION 99.0
4ZL9 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal III PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 98.4
5TLQ OXIDOREDUCTASE 10/11/16 Model structure of the oxidized PaDsbA1 and 3-[(2-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-4-amine complex PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0
4ZL8 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal II PSEUDOMONAS AERUGINOSA 1.395 X-RAY DIFFRACTION 98.4
5DCH OXIDOREDUCTASE 08/24/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I in complex with MIPS-0000851 (3-[(2-METHYLBENZYL)SULFANYL]-4H-1,2,4-TRIAZOL-4-AMINE) PSEUDOMONAS AERUGINOSA 1.447 X-RAY DIFFRACTION 98.4
2MBT OXIDOREDUCTASE 08/03/13 NMR study of PaDsbA PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 149 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005029 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5489
Search term: dsbA

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Cloning and expression of the gene for a protein disulfide oxidoreductase from Azotobacter vinelandii: complementation of an Escherichia coli dsbA mutant strain.
Ng TC, Kwik JF, Maier RJ
Gene 1997 Mar;188(1):109-13
PubMed ID: 9099867
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994