Pseudomonas aeruginosa PAO1, PA5496 (nrdJb)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5496
Name
nrdJb
Replicon chromosome
Genomic location 6188166 - 6188855 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentnrdJa and nrdJb are the subunits that form a functional Class II ribonucleotide reductase

Cross-References

RefSeq NP_254183.1
GI 15600689
Affymetrix PA5496_at
DNASU PaCD00008368
Entrez 877839
GenBank AAG08881.1
INSDC AAG08881.1
NCBI Locus Tag PA5496
protein_id(GenBank) gb|AAG08881.1|AE004962_5|gnl|PseudoCAP|PA5496
TIGR NTL03PA05497
UniParc UPI00000C6037
UniProtKB Acc Q9HT77
UniProtKB ID Q9HT77_PSEAE
UniRef100 UniRef100_Q9HT77
UniRef50 UniRef50_U2EMP0
UniRef90 UniRef90_Q9HT77

Product

Feature Type CDS
Coding Frame 1
Product Name
class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJb
Product Name Confidence: Class 1
Synonyms NrdJb (changed from hypothetical protein)
class II (cobalamin-dependent) ribonucleotide-diphosphate reductase subunit, NrdJb
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.01
Kyte-Doolittle Hydrophobicity Value -0.043
Molecular Weight (kDa) 24.9
Isoelectric Point (pI) 6.35

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 79 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005035 (341 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5496
Search term: nrdJb

References

Two proteins mediate class II ribonucleotide reductase activity in Pseudomonas aeruginosa: expression and transcriptional analysis of the aerobic enzymes.
Torrents E, Poplawski A, Sjöberg BM
J. Biol. Chem. 2005 Apr;280(17):16571-8
PubMed ID: 15722359