Pseudomonas aeruginosa PAO1, PA5542

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5542
Name
Replicon chromosome
Genomic location 6234500 - 6235744 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254229.1
GI 15600735
Affymetrix PA5542_at
Entrez 878466
GenBank AAG08927.1
INSDC AAG08927.1
NCBI Locus Tag PA5542
protein_id(GenBank) gb|AAG08927.1|AE004966_9|gnl|PseudoCAP|PA5542
TIGR NTL03PA05543
UniParc UPI00000C6064
UniProtKB Acc Q9HT32
UniProtKB ID Q9HT32_PSEAE
UniRef100 UniRef100_Q9HT32
UniRef50 UniRef50_F6AJR1
UniRef90 UniRef90_Q9HT32

Product

Feature Type CDS
Coding Frame 1
Product Name
Pseudomonas imipenem beta-lactamase PIB-1
Product Name Confidence: Class 1
Synonyms hypothetical protein
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.58
Kyte-Doolittle Hydrophobicity Value -0.316
Molecular Weight (kDa) 45.9
Isoelectric Point (pI) 6.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 255 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005074 (430 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5542

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of a novel Zn²⁺-dependent intrinsic imipenemase from Pseudomonas aeruginosa.
Fajardo A, Hernando-Amado S, Oliver A, Ball G, Filloux A, Martinez JL
J. Antimicrob. Chemother. 2014 Nov;69(11):2972-8
PubMed ID: 25185138