Pseudomonas aeruginosa PAO1, PA5558 (atpF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5558
Name
atpF
Synonym: uncF
Synonym: papF
Replicon chromosome
Genomic location 6252707 - 6253177 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254245.1
GI 15600751
Affymetrix PA5558_atpF_at
DNASU PaCD00007316
Entrez 878510
GenBank AAG08943.1
INSDC AAG08943.1
NCBI Locus Tag PA5558
protein_id(GenBank) gb|AAG08943.1|AE004967_14|gnl|PseudoCAP|PA5558
TIGR NTL03PA05559
UniParc UPI00000C6072
UniProtKB Acc Q9HT16
UniProtKB ID ATPF_PSEAE
UniRef100 UniRef100_Q02DF0
UniRef50 UniRef50_B0U5A2
UniRef90 UniRef90_A6VF36

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP synthase B chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (16.96 kDa, PMID:21751344)
Charge (pH 7) -1.55
Kyte-Doolittle Hydrophobicity Value -0.154
Molecular Weight (kDa) 16.9
Isoelectric Point (pI) 5.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 395 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005089 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5558
Search term: atpF

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Targeted mutagenesis of the b subunit of F1F0 ATP synthase in Escherichia coli: Glu-77 through Gln-85.
McCormick KA, Cain BD
J Bacteriol 1991 Nov;173(22):7240-8
PubMed ID: 1682301
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344