Pseudomonas aeruginosa PAO1, PA0090 (clpV1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0090
Name
clpV1
Replicon chromosome
Genomic location 108221 - 110929 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_248780.1
GI 15595288
Affymetrix PA0090_at
Entrez 879553
GenBank AAG03480.1
INSDC AAG03480.1
NCBI Locus Tag PA0090
protein_id(GenBank) gb|AAG03480.1|AE004447_13|gnl|PseudoCAP|PA0090
TIGR NTL03PA00091
UniParc UPI00000C4F2B
UniProtKB Acc Q9I742
UniProtKB ID CLPV1_PSEAE
UniRef100 UniRef100_Q9I742
UniRef50 UniRef50_Q9I742
UniRef90 UniRef90_Q9I742

Product

Feature Type CDS
Coding Frame 1
Product Name
ClpV1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -21.22
Kyte-Doolittle Hydrophobicity Value -0.156
Molecular Weight (kDa) 98.7
Isoelectric Point (pI) 5.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database Victors : 4231
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575
Source 2
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 597 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000089 (1211 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0090
Search term: clpV1
Search term: ClpV1

Human Homologs

Ensembl 86, assembly GRCh38.p7
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 8.0e-35
Percent Identity: 31.3

References

A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus.
Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordoñez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ
Science 2006 Jun;312(5779):1526-30
PubMed ID: 16763151
A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan;7(1):25-37
PubMed ID: 20114026