Pseudomonas aeruginosa PAO1, PA0247 (pobA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0247
Name
pobA
Replicon chromosome
Genomic location 279574 - 280758 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248938.1
GI 15595444
Affymetrix PA0247_pobA_at
Entrez 882128
GenBank AAG03636.1
INSDC AAG03636.1
NCBI Locus Tag PA0247
protein_id(GenBank) gb|AAG03636.1|AE004463_1|gnl|PseudoCAP|PA0247
TIGR NTL03PA00248
UniParc UPI0000110EFA
UniProtKB Acc P20586
UniProtKB ID PHHY_PSEAE
UniRef100 UniRef100_P20586
UniRef50 UniRef50_P20586
UniRef90 UniRef90_P20586

Product

Feature Type CDS
Coding Frame 1
Product Name
p-hydroxybenzoate hydroxylase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.97
Kyte-Doolittle Hydrophobicity Value -0.258
Molecular Weight (kDa) 44.3
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1DOC OXIDOREDUCTASE 09/06/94 THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1PDH OXIDOREDUCTASE 12/01/94 CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE RECONSTITUTED WITH THE MODIFIED FAD PRESENT IN ALCOHOL OXIDASE FROM METHYLOTROPHIC YEASTS: EVIDENCE FOR AN ARABINOFLAVIN PSEUDOMONAS FLUORESCENS 2.1 X-RAY DIFFRACTION 99.5
1DOD OXIDOREDUCTASE 09/06/94 THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0
1YKJ OXIDOREDUCTASE 01/18/05 A45G p-hydroxybenzoate hydroxylase with p-hydroxybenzoate bound PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 99.7
1IUT OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 7.4 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1DOE OXIDOREDUCTASE 09/06/94 THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0
1PBE OXIDOREDUCTASE 07/06/94 CRYSTAL STRUCTURE OF THE P-HYDROXYBENZOATE HYDROXYLASE-SUBSTRATE COMPLEX REFINED AT 1.9 ANGSTROMS RESOLUTION. ANALYSIS OF THE ENZYME-SUBSTRATE AND ENZYME-PRODUCT COMPLEXES PSEUDOMONAS FLUORESCENS 1.9 X-RAY DIFFRACTION 99.5
2PHH OXIDOREDUCTASE 06/19/89 THE COENZYME ANALOGUE ADENOSINE 5-DIPHOSPHORIBOSE DISPLACES FAD IN THE ACTIVE SITE OF P-HYDROXYBENZOATE HYDROXYLASE. AN X-RAY CRYSTALLOGRAPHIC INVESTIGATION PSEUDOMONAS FLUORESCENS 2.7 X-RAY DIFFRACTION 99.5
1PXC OXIDOREDUCTASE 09/27/94 CRYSTAL STRUCTURES OF MUTANT PSEUDOMONAS AERUGINOSA P-HYDROXYBENZOATE HYDROXYLASE: THE TYR201PHE, TYR385PHE, AND ASN300ASP VARIANTS PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 99.7
1PXA OXIDOREDUCTASE 09/27/94 CRYSTAL STRUCTURES OF MUTANT PSEUDOMONAS AERUGINOSA P-HYDROXYBENZOATE HYDROXYLASE: THE TYR201PHE, TYR385PHE, AND ASN300ASP VARIANTS PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.7
1IUV OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 5.0 PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0
1IUX OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 9.4 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1K0L HYDROLASE 09/19/01 Pseudomonas aeruginosa phbh R220Q free of p-OHB PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 99.7
1DOB OXIDOREDUCTASE 09/06/94 THE MOBIL FLAVIN OF 4-OH BENZOATE HYDROXYLASE: MOTION OF A PROSTHETIC GROUP REGULATES CATALYSIS PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 99.7
1IUW OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-4-HYDROXYBENZOATE AT PH 7.4 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1PXB OXIDOREDUCTASE 09/27/94 CRYSTAL STRUCTURES OF MUTANT PSEUDOMONAS AERUGINOSA P-HYDROXYBENZOATE HYDROXYLASE: THE TYR201PHE, TYR385PHE, AND ASN300ASP VARIANTS PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 99.7
1D7L OXIDOREDUCTASE 10/18/99 STRUCTURE-FUNCTION CORRELATIONS OF THE REACTION OF REDUCED NICOTINAMIDE ANALOGS WITH P-HYDROXYBENZOATE HYDROXYLASE SUBSTITUTED WITH A SERIES OF 8-SUBSTITUTED FLAVINS PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0
1K0I HYDROLASE 09/19/01 Pseudomonas aeruginosa phbh R220Q in complex with 100mM PHB PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 99.7
1IUU OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 9.4 PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0
1PBC OXIDOREDUCTASE 07/06/94 CRYSTAL STRUCTURES OF WILD-TYPE P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE, 2,4-DIHYDROXYBENZOATE AND 2-HYDROXY-4-AMINOBENZOATE AND OF THE TRY222ALA MUTANT, COMPLEXED WITH 2-HYDROXY-4-AMINOBENZOATE. EVIDENCE FOR A PROTON CHANNEL AND A NEW BINDING MODE OF THE FLAVIN RING PSEUDOMONAS FLUORESCENS 2.8 X-RAY DIFFRACTION 99.2
1K0J HYDROLASE 09/19/01 Pseudomonas aeruginosa phbh R220Q in complex with NADPH and free of p-OHB PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 99.7
1PBB OXIDOREDUCTASE 07/06/94 CRYSTAL STRUCTURES OF WILD-TYPE P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE, 2,4-DIHYDROXYBENZOATE AND 2-HYDROXY-4-AMINOBENZOATE AND OF THE TRY222ALA MUTANT, COMPLEXED WITH 2-HYDROXY-4-AMINOBENZOATE. EVIDENCE FOR A PROTON CHANNEL AND A NEW BINDING MODE OF THE FLAVIN RING PSEUDOMONAS FLUORESCENS 2.5 X-RAY DIFFRACTION 99.2
1PHH OXIDOREDUCTASE 11/04/87 CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH ITS REACTION PRODUCT 3,4-DIHYDROXYBENZOATE PSEUDOMONAS FLUORESCENS 2.3 X-RAY DIFFRACTION 99.5
1IUS OXIDOREDUCTASE 11/22/95 P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH 4-AMINOBENZOATE AT PH 5.0 PSEUDOMONAS AERUGINOSA 2.2 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
8-Demethyl-8-Dimethylamino-Flavin-Adenine-Dinucleotide DB03482 DrugBank 5.1.4 P20586 p-hydroxybenzoate hydroxylase 100.0 394 6.0e-229
Aminobenzoic acid DB02362 DrugBank 5.1.4 P20586 p-hydroxybenzoate hydroxylase 100.0 394 6.0e-229
Flavin adenine dinucleotide DB03147 DrugBank 5.1.4 P20586 p-hydroxybenzoate hydroxylase 100.0 394 6.0e-229
2,4-Dihydroxybenzoic Acid DB02839 DrugBank 5.1.4 P20586 p-hydroxybenzoate hydroxylase 100.0 394 6.0e-229
P-Hydroxybenzoic Acid DB04242 DrugBank 5.1.4 P20586 p-hydroxybenzoate hydroxylase 100.0 394 6.0e-229

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 151 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000245 (514 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0247
Search term: pobA

References

Sequence and organization of pobA, the gene coding for p-hydroxybenzoate hydroxylase, an inducible enzyme from Pseudomonas aeruginosa.
Entsch B, Nan Y, Weaich K, Scott KF
Gene 1988 Nov 30;71(2):279-91
PubMed ID: 2465205
pH-dependent structural changes in the active site of p-hydroxybenzoate hydroxylase point to the importance of proton and water movements during catalysis.
Gatti DL, Entsch B, Ballou DP, Ludwig ML
Biochemistry 1996 Jan;35(2):567-78
PubMed ID: 8555229