Pseudomonas aeruginosa PAO1, PA0260 (tle3)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0260
Name
tle3
Replicon chromosome
Genomic location 291154 - 293304 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentTle3 is an antibacterial T6SS effector. Toxic when delivered into the periplasm of prey bacteria. Tle3 is dependent on the H2-T6SS machinery , Tla3 and VgrG2b for its secretion. Tli3 neutralizes its antibacterial activity.

Cross-References

RefSeq NP_248951.1
GI 15595457
Affymetrix PA0260_at
Entrez 879499
GenBank AAG03649.1
INSDC AAG03649.1
NCBI Locus Tag PA0260
protein_id(GenBank) gb|AAG03649.1|AE004464_2|gnl|PseudoCAP|PA0260
TIGR NTL03PA00261
UniParc UPI00000C4FC9
UniProtKB Acc Q9I6M9
UniProtKB ID Q9I6M9_PSEAE
UniRef100 UniRef100_Q9I6M9
UniRef50 UniRef50_Q9I6M9
UniRef90 UniRef90_Q9I6M9

Product

Feature Type CDS
Coding Frame 1
Product Name
Type 6 lipase effector, Tle3
Product Name Confidence: Class 1
Synonyms Tle3
Evidence for Translation
Charge (pH 7) -0.33
Kyte-Doolittle Hydrophobicity Value -0.370
Molecular Weight (kDa) 78.2
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 17 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000258 (272 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0260
Search term: tle3

Human Homologs

References

Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors.
Russell AB, LeRoux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD
Nature 2013 Apr 25;496(7446):508-12
PubMed ID: 23552891
Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network.
Sana TG, Baumann C, Merdes A, Soscia C, Rattei T, Hachani A, Jones C, Bennett KL, Filloux A, Superti-Furga G, Voulhoux R, Bleves S
mBio 2015 Jun 2;6(3):e00712
PubMed ID: 26037124
A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa.
Berni B, Soscia C, Djermoun S, Ize B, Bleves S
Front Microbiol 2019;10:1218
PubMed ID: 31231326