Pseudomonas aeruginosa PAO1, PA0262 (vgrG2b)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0262
Name	vgrG2b
Replicon	chromosome
Genomic location	293802 - 296861 (- strand)
Transposon Mutants	13 transposon mutants in PAO1
Transposon Mutants in orthologs	10 transposon mutants in orthologs
Comment	Tli3 neutralizes the antibacterial activity of VgrG2b (PMID:31231326).
Comment	H2-T6SS evolved effector demonstrating antibacterial and anti-eukaryotic activity (trans-kingdom toxin) delivered into epithelial cells through the H2-T6SS machinery and VgrG2a (PA1511)
Comment	Required for Tle3 secretion, the C-Terminal domain of VgrG2b is antibacterial when delivered into the periplasm of prey bacteria (in the heterologous host E. coli) and its toxicity relies on an intact protease catalytic site (residus 932-941, PFAM04298)
Comment	The C-Terminal domain of VgrG2b interacts with the gamma-tubulin complex to promote a microtubule-dependant internalization of P. aeruginosa into non phagocytic cells (PMID:26037124)

Cross-References

RefSeq	NP_248953.1
GI	15595459
Affymetrix	PA0262_at
Entrez	881981
GenBank	AAG03651.1
INSDC	AAG03651.1
NCBI Locus Tag	PA0262
protein_id(GenBank)	gb|AAG03651.1|AE004464_4|gnl|PseudoCAP|PA0262
TIGR	NTL03PA00263
UniParc	UPI00000C4FCB
UniProtKB Acc	Q9I6M7
UniProtKB ID	Q9I6M7_PSEAE
UniRef100	UniRef100_Q9I6M7
UniRef50	UniRef50_Q9I3K1
UniRef90	UniRef90_Q9I3K1

Product

Feature Type	CDS
Coding Frame	1
Product Name	VgrG2b Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-19.34
Kyte-Doolittle Hydrophobicity Value	-0.478
Molecular Weight (kDa)	113.0
Isoelectric Point (pI)	6.16

Subcellular localization

Individual Mappings	Localization Confidence PMID Host Associated Class 1 26037124 Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6H56	METAL BINDING PROTEIN	07/23/18	Effector domain of Pseudomonas aeruginosa VgrG2b	Pseudomonas aeruginosa PAO1	3.2	X-RAY DIFFRACTION	98.8

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 146 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000090 (3342 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0262 Search term: vgrG2b Search term: VgrG2b

Human Homologs

References

Rhs elements comprise three subfamilies which diverged prior to acquisition by Escherichia coli. Wang YD, Zhao S, Hill CW J Bacteriol 1998 Aug;180(16):4102-10 PubMed ID: 9696756

Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jové D, Filloux A J Biol Chem 2011 Apr 8;286(14):12317-27 PubMed ID: 21325275

Internalization of Pseudomonas aeruginosa Strain PAO1 into Epithelial Cells Is Promoted by Interaction of a T6SS Effector with the Microtubule Network. Sana TG, Baumann C, Merdes A, Soscia C, Rattei T, Hachani A, Jones C, Bennett KL, Filloux A, Superti-Furga G, Voulhoux R, Bleves S mBio 2015 Jun 2;6(3):e00712 PubMed ID: 26037124

A Type VI Secretion System Trans-Kingdom Effector Is Required for the Delivery of a Novel Antibacterial Toxin in Pseudomonas aeruginosa. Berni B, Soscia C, Djermoun S, Ize B, Bleves S Front Microbiol 2019;10:1218 PubMed ID: 31231326