Pseudomonas aeruginosa PAO1, PA0291 (oprE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0291
Name
oprE
Replicon chromosome
Genomic location 327284 - 328666 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_248982.1
GI 15595488
Affymetrix PA0291_oprE_at
Entrez 879473
GenBank AAG03680.1
INSDC AAG03680.1
NCBI Locus Tag PA0291
protein_id(GenBank) gb|AAG03680.1|AE004467_2|gnl|PseudoCAP|PA0291
TIGR NTL03PA00292
UniParc UPI00000D428C
UniProtKB Acc G3XDA5
UniProtKB ID G3XDA5_PSEAE
UniRef100 UniRef100_G3XDA5
UniRef50 UniRef50_G3XDA5
UniRef90 UniRef90_G3XDA5

Product

Feature Type CDS
Coding Frame 1
Product Name
Anaerobically-induced outer membrane porin OprE precursor
Product Name Confidence: Class 1
Synonyms Porin E1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (49.67 kDa, PMID:21751344)
Charge (pH 7) 4.20
Kyte-Doolittle Hydrophobicity Value -0.436
Molecular Weight (kDa) 49.7
Isoelectric Point (pI) 9.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Vesicle Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4FRX TRANSPORT PROTEIN 06/26/12 Crystal Structure of Pseudomonas aeruginosa OccK8 (OprE) PSEUDOMONAS AERUGINOSA 1.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 19 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000288 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0291
Search term: oprE

References

Identification of porins in the outer membrane of Pseudomonas aeruginosa that form small diffusion pores.
Yoshihara E, Nakae T
J Biol Chem 1989 Apr 15;264(11):6297-301
PubMed ID: 2539376
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878