Pseudomonas aeruginosa PAO1, PA0328 (aaaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0328
Name
aaaA
Replicon chromosome
Genomic location 368462 - 370405 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249019.1
GI 15595525
Affymetrix PA0328_at
Entrez 882183
GenBank AAG03717.1
INSDC AAG03717.1
NCBI Locus Tag PA0328
protein_id(GenBank) gb|AAG03717.1|AE004470_11|gnl|PseudoCAP|PA0328
TIGR NTL03PA00329
UniParc UPI00000C500B
UniProtKB Acc Q9I6G3
UniProtKB ID Q9I6G3_PSEAE
UniRef100 UniRef100_Q9I6G3
UniRef50 UniRef50_Q9I6G3
UniRef90 UniRef90_Q9I6G3

Product

Feature Type CDS
Coding Frame 1
Product Name
arginine-specific autotransporter of Pseudomonas aeruginosa, AaaA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (70.36 kDa, PMID:21751344)
Charge (pH 7) -25.65
Kyte-Doolittle Hydrophobicity Value -0.412
Molecular Weight (kDa) 70.3
Isoelectric Point (pI) 4.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category Not available / Not known
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Mouse chronic wound Infection model
Reference 22927813

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 164 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000320 (339 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0328
Search term: aaaA

References

The DNA sequence of the sulfate activation locus from Escherichia coli K-12.
Leyh TS, Vogt TF, Suo Y
J Biol Chem 1992 May 25;267(15):10405-10
PubMed ID: 1316900
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
Luckett JC, Darch O, Watters C, Abuoun M, Wright V, Paredes-Osses E, Ward J, Goto H, Heeb S, Pommier S, Rumbaugh KP, Cámara M, Hardie KR
PLoS Pathog. 2012 ;8(8):e1002854
PubMed ID: 22927813