Pseudomonas aeruginosa PAO1, PA0381 (thiG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0381
Name
thiG
Replicon chromosome
Genomic location 423719 - 424516 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249072.1
GI 15595578
Affymetrix PA0381_thiG_at
DNASU PaCD00006163
Entrez 879802
GenBank AAG03770.1
INSDC AAG03770.1
NCBI Locus Tag PA0381
protein_id(GenBank) gb|AAG03770.1|AE004475_12|gnl|PseudoCAP|PA0381
TIGR NTL03PA00382
UniParc UPI0000136DF9
UniProtKB Acc Q9I6B4
UniProtKB ID THIG_PSEAE
UniRef100 UniRef100_B7V3W7
UniRef50 UniRef50_O31618
UniRef90 UniRef90_C1DI61

Product

Feature Type CDS
Coding Frame 1
Product Name
thiamine biosynthesis protein, thiazole moiety
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.43
Kyte-Doolittle Hydrophobicity Value 0.182
Molecular Weight (kDa) 28.2
Isoelectric Point (pI) 4.75

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1WV2 BIOSYNTHETIC PROTEIN 12/10/04 Crystal structure of thiamine biosynthesis protein from Pseudomonas Aeruginosa PSEUDOMONAS AERUGINOSA 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 420 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000372 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0381
Search term: thiG

References

Structural genes for thiamine biosynthetic enzymes (thiCEFGH) in Escherichia coli K-12.
Vander Horn PB, Backstrom AD, Stewart V, Begley TP
J. Bacteriol. 1993 Feb;175(4):982-92
PubMed ID: 8432721
Expression of thiamin biosynthetic genes (thiCOGE) and production of symbiotic terminal oxidase cbb3 in Rhizobium etli.
Miranda-Ríos J, Morera C, Taboada H, Dávalos A, Encarnación S, Mora J, Soberón M
J. Bacteriol. 1997 Nov;179(22):6887-93
PubMed ID: 9371431