Pseudomonas aeruginosa PAO1, PA0395 (pilT)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 436570 - 437604 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249086.1
GI 15595592
Affymetrix PA0395_pilT_at
DNASU PaCD00006170
Entrez 878389
GenBank AAG03784.1
INSDC AAG03784.1
NCBI Locus Tag PA0395
protein_id(GenBank) gb|AAG03784.1|AE004477_1|gnl|PseudoCAP|PA0395
UniParc UPI0000131AD1
UniProtKB Acc P24559
UniRef100 UniRef100_P24559
UniRef50 UniRef50_P24559
UniRef90 UniRef90_P24559


Feature Type CDS
Coding Frame 1
Product Name
twitching motility protein PilT
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilT
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.64
Kyte-Doolittle Hydrophobicity Value -0.114
Molecular Weight (kDa) 38.0
Isoelectric Point (pI) 6.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3JVU ATP binding protein 09/17/09 Crystal structure of unliganded P. aeruginosa PilT PSEUDOMONAS AERUGINOSA 3.1 X-RAY DIFFRACTION 100.0
3JVV ATP binding protein 09/17/09 Crystal Structure of P. aeruginosa PilT with bound AMP-PCP PSEUDOMONAS AERUGINOSA 2.6 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Host Organism
Reference 9224879
Source 2
Database PseudoCAP
Category Type IV pili biosynthesis
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Galleria mellonella
Notes Mortality greater after inoculation with wild-type PAO1 compared with pilT mutant
Infection model Galleria mellonella killing assay
Reference 16849183

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000386 (552 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0395
Search term: pilT


Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria.
Whitchurch CB, Hobbs M, Livingston SP, Krishnapillai V, Mattick JS
Gene 1991 May 15;101(1):33-44
PubMed ID: 1676385
PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa.
Hobbs M, Collie ES, Free PD, Livingston SP, Mattick JS
Mol. Microbiol. 1993 Mar;7(5):669-82
PubMed ID: 8097014
Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa.
Whitchurch CB, Mattick JS
Mol. Microbiol. 1994 Sep;13(6):1079-91
PubMed ID: 7854122
Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia.
Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN
Infect. Immun. 1999 Jul;67(7):3625-30
PubMed ID: 10377148