Pseudomonas aeruginosa PAO1, PA0396 (pilU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0396
Name
pilU
Replicon chromosome
Genomic location 437782 - 438930 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249087.1
GI 15595593
Affymetrix PA0396_pilU_at
Entrez 878362
GenBank AAG03785.1
INSDC AAG03785.1
NCBI Locus Tag PA0396
protein_id(GenBank) gb|AAG03785.1|AE004477_2|gnl|PseudoCAP|PA0396
TIGR NTL03PA00397
UniParc UPI00000D420E
UniProtKB Acc G3XCX3
UniProtKB ID G3XCX3_PSEAE
UniRef100 UniRef100_W0YLF7
UniRef50 UniRef50_Q8P759
UniRef90 UniRef90_A6UYK6

Product

Feature Type CDS
Coding Frame 1
Product Name
twitching motility protein PilU
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilU
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.19
Kyte-Doolittle Hydrophobicity Value -0.236
Molecular Weight (kDa) 42.5
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili biosynthesis
Evidence
Host Organism
Notes
Reference 9224879

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 524 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000387 (423 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0396
Search term: pilU

References

Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa.
Whitchurch CB, Mattick JS
Mol. Microbiol. 1994 Sep;13(6):1079-91
PubMed ID: 7854122
Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia.
Comolli JC, Hauser AR, Waite L, Whitchurch CB, Mattick JS, Engel JN
Infect. Immun. 1999 Jul;67(7):3625-30
PubMed ID: 10377148