Pseudomonas aeruginosa PAO1, PA0402 (pyrB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0402
Name
pyrB
Replicon chromosome
Genomic location 444687 - 445691 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249093.1
GI 15595599
Affymetrix PA0402_pyrB_at
Entrez 878267
GenBank AAG03791.1
INSDC AAG03791.1
NCBI Locus Tag PA0402
protein_id(GenBank) gb|AAG03791.1|AE004477_8|gnl|PseudoCAP|PA0402
TIGR NTL03PA00403
UniParc UPI0000132C39
UniProtKB Acc Q59653
UniProtKB ID PYRB_PSEAE
UniRef100 UniRef100_B7V3Y9
UniRef50 UniRef50_Q59711
UniRef90 UniRef90_B7V3Y9

Product

Feature Type CDS
Coding Frame 1
Product Name
aspartate carbamoyltransferase
Product Name Confidence: Class 2
Synonyms aspartate transcarbamoylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -0.89
Kyte-Doolittle Hydrophobicity Value -0.114
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 6.80

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 646 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000393 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0402
Search term: pyrB

References

Aspartate transcarbamoylase genes of Pseudomonas putida: requirement for an inactive dihydroorotase for assembly into the dodecameric holoenzyme.
Schurr MJ, Vickrey JF, Kumar AP, Campbell AL, Cunin R, Benjamin RC, Shanley MS, O'Donovan GA
J. Bacteriol. 1995 Apr;177(7):1751-9
PubMed ID: 7896697
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994