Pseudomonas aeruginosa PAO1, PA0407 (gshB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0407
Name
gshB
Replicon chromosome
Genomic location 448431 - 449384 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249098.1
GI 15595604
Affymetrix PA0407_gshB_at
Entrez 878223
GenBank AAG03796.1
INSDC AAG03796.1
NCBI Locus Tag PA0407
protein_id(GenBank) gb|AAG03796.1|AE004478_1|gnl|PseudoCAP|PA0407
TIGR NTL03PA00408
UniParc UPI000012BBCB
UniProtKB Acc Q9I697
UniProtKB ID GSHB_PSEAE
UniRef100 UniRef100_Q9I697
UniRef50 UniRef50_P04425
UniRef90 UniRef90_Q9I697

Product

Feature Type CDS
Coding Frame 1
Product Name
glutathione synthetase
Product Name Confidence: Class 2
Synonyms GSH-II
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -3.91
Kyte-Doolittle Hydrophobicity Value -0.282
Molecular Weight (kDa) 35.7
Isoelectric Point (pI) 5.76

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 270 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000398 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0407
Search term: gshB

Human Homologs

Ensembl 97, assembly GRCh38.p12
ribosomal modification protein rimK like family member A [Source:HGNC Symbol;Acc:HGNC:28725]
E-value: 2.9e-09
Percent Identity: 28.1

References

Purification and characterization of glutathione synthetase from Escherichia coli B.
Gushima H, Miya T, Murata K, Kimura A
J Appl Biochem 1983 Jun;5(3):210-8
PubMed ID: 6389479
Role of cysteine residues in glutathione synthetase from Escherichia coli B. Chemical modification and oligonucleotide site-directed mutagenesis.
Kato H, Tanaka T, Nishioka T, Kimura A, Oda J
J Biol Chem 1988 Aug 25;263(24):11646-51
PubMed ID: 3042775
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994