Pseudomonas aeruginosa PAO1, PA0408 (pilG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0408
Name
pilG
Replicon chromosome
Genomic location 449639 - 450046 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249099.1
GI 15595605
Affymetrix PA0408_pilG_at
DNASU PaCD00006176
Entrez 878203
GenBank AAG03797.1
INSDC AAG03797.1
NCBI Locus Tag PA0408
protein_id(GenBank) gb|AAG03797.1|AE004478_2|gnl|PseudoCAP|PA0408
TIGR NTL03PA00409
UniParc UPI0000131AC9
UniProtKB Acc P46384
UniProtKB ID PILG_PSEAE
UniRef100 UniRef100_P46384
UniRef50 UniRef50_P46384
UniRef90 UniRef90_P46384

Product

Feature Type CDS
Coding Frame 1
Product Name
twitching motility protein PilG
Product Name Confidence: Class 1
Synonyms type 4 fimbrial biogenesis protein PilG
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.41
Kyte-Doolittle Hydrophobicity Value 0.001
Molecular Weight (kDa) 14.7
Isoelectric Point (pI) 7.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0082
Category Type IV pili twitching motility related proteins
Evidence
Host Organism
Notes
Reference 9224879

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 623 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000399 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0408
Search term: pilG

References

The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.
Darzins A
J. Bacteriol. 1993 Sep;175(18):5934-44
PubMed ID: 8104179
The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa--a review.
Mattick JS, Whitchurch CB, Alm RA
Gene 1996 Nov;179(1):147-55
PubMed ID: 8955641
Molecular genetic analysis of type-4 pilus biogenesis and twitching motility using Pseudomonas aeruginosa as a model system--a review.
Darzins A, Russell MA
Gene 1997 Jun;192(1):109-15
PubMed ID: 9224880