Pseudomonas aeruginosa PAO1, PA0424 (mexR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0424
Name
mexR
Replicon chromosome
Genomic location 471306 - 471749 (- strand)
Transposon Mutants 3 transposon mutants in PAO1

Cross-References

RefSeq NP_249115.1
GI 15595621
Affymetrix PA0424_mexR_at
DNASU PaCD00006181
Entrez 877857
GenBank AAG03813.1
INSDC AAG03813.1
NCBI Locus Tag PA0424
protein_id(GenBank) gb|AAG03813.1|AE004479_8|gnl|PseudoCAP|PA0424
TIGR NTL03PA00425
UniParc UPI000012F023
UniProtKB Acc P52003
UniProtKB ID MEXR_PSEAE
UniRef100 UniRef100_P52003
UniRef50 UniRef50_P52003
UniRef90 UniRef90_P52003

Product

Feature Type CDS
Coding Frame 1
Product Name
multidrug resistance operon repressor MexR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -3.65
Kyte-Doolittle Hydrophobicity Value -0.355
Molecular Weight (kDa) 17.0
Isoelectric Point (pI) 5.92

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexR MexR is the repressor of the MexRAB-OprM operon. Mutant forms of mexR result in up-regulation of efflux pump system MexAB-OprM. ARO:3000506 protein overexpression model Strict 295.0 100.0 3.0.1

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4ZZL TRANSCRIPTION 04/10/15 MexR R21W derepressor mutant causing multidrug resistance in P. aeruginosa by mexAB-oprM efflux pump expression PSEUDOMONAS AERUGINOSA 2.19 X-RAY DIFFRACTION 99.3
3MEX Transcription, Transcription Regulation 04/01/10 Crystal structure of MexR in oxidized state PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 99.3
3ECH Transcription, Transcription regulation 08/30/08 The MarR-family repressor MexR in complex with its antirepressor ArmR PSEUDOMONAS AERUGINOSA 1.802 X-RAY DIFFRACTION 98.6
1LNW TRANSCRIPTION REPRESSOR 05/03/02 CRYSTAL STRUCTURE OF THE MEXR REPRESSOR OF THE MEXAB-OPRM MULTIDRUG EFFLUX OPERON OF PSEUDOMONAS AERUGINOSA PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 211 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000415 (302 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0424
Search term: mexR

References

Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression.
Poole K, Tetro K, Zhao Q, Neshat S, Heinrichs DE, Bianco N
Antimicrob. Agents Chemother. 1996 Sep;40(9):2021-8
PubMed ID: 8878574