Pseudomonas aeruginosa PAO1, PA0426 (mexB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0426
Name
mexB
Replicon chromosome
Genomic location 473191 - 476331 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs

Cross-References

RefSeq NP_249117.1
GI 15595623
Affymetrix PA0426_mexB_at
Entrez 877852
GenBank AAG03815.1
INSDC AAD45628.1
NCBI Locus Tag PA0426
protein_id(GenBank) gb|AAG03815.1|AE004479_10|gnl|PseudoCAP|PA0426
TIGR NTL03PA00427
UniParc UPI000012F022
UniProtKB Acc P52002
UniProtKB ID MEXB_PSEAE
UniRef100 UniRef100_P52002
UniRef50 UniRef50_P52002
UniRef90 UniRef90_P52002

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexB
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter MexB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (112.79 kDa, PMID:21751344)
Charge (pH 7) -9.89
Kyte-Doolittle Hydrophobicity Value 0.274
Molecular Weight (kDa) 112.8
Isoelectric Point (pI) 5.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
adeF AdeF is the membrane fusion protein of the multidrug efflux complex AdeFGH. ARO:3000777 protein homolog model Strict 762.7 41.62 3.0.1

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3W9J MEMBRANE PROTEIN 04/04/13 Structural basis for the inhibition of bacterial multidrug exporters PSEUDOMONAS AERUGINOSA 3.15 X-RAY DIFFRACTION 100.0
3W9I MEMBRANE PROTEIN 04/04/13 Structural basis for the inhibition of bacterial multidrug exporters PSEUDOMONAS AERUGINOSA 2.71 X-RAY DIFFRACTION 100.0
2V50 MEMBRANE PROTEIN 10/01/08 The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB PSEUDOMONAS AERUGINOSA PA01 3.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2150 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0426
Search term: mexB

References

Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine.
Poole K, Heinrichs DE, Neshat S
Mol. Microbiol. 1993 Nov;10(3):529-44
PubMed ID: 7968531
Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa.
Li XZ, Nikaido H, Poole K
Antimicrob. Agents Chemother. 1995 Sep;39(9):1948-53
PubMed ID: 8540696
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344