Pseudomonas aeruginosa PAO1, PA0459

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0459
Name
Synonym: clpC
Replicon chromosome
Genomic location 518083 - 520635 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_249150.1
GI 15595656
Affymetrix PA0459_at
Entrez 881659
GenBank AAG03848.1
NCBI Locus Tag PA0459
protein_id(GenBank) gb|AAG03848.1|AE004483_6|gnl|PseudoCAP|PA0459
TIGR NTL03PA00460
UniProtKB Acc Q9I659
UniProtKB ID Q9I659_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
probable ClpA/B protease ATP binding subunit
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.30
Kyte-Doolittle Hydrophobicity Value -0.415
Molecular Weight (kDa) 94.1
Isoelectric Point (pI) 6.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000451 (879 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0459

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 5.2e-50
Percent Identity: 36.3

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 5.2e-50
Percent Identity: 36.3

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 5.2e-50
Percent Identity: 36.3

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 5.2e-50
Percent Identity: 36.3

Human Homologs

Ensembl 97, assembly GRCh38.p12
ClpB homolog, mitochondrial AAA ATPase chaperonin [Source:HGNC Symbol;Acc:HGNC:30664]
E-value: 5.2e-50
Percent Identity: 36.3

References

Stress induction of clpC in Bacillus subtilis and its involvement in stress tolerance.
Krüger E, Völker U, Hecker M
J. Bacteriol. 1994 Jun;176(11):3360-7
PubMed ID: 8195092
LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan;96
PubMed ID: 24291602