Pseudomonas aeruginosa PAO1, PA0482 (glcB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0482
Name
glcB
Synonym: aceB
Replicon chromosome
Genomic location 542170 - 544347 (- strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249173.1
GI 15595679
Affymetrix PA0482_glcB_at
Entrez 879477
GenBank AAG03871.1
INSDC AAG03871.1
NCBI Locus Tag PA0482
protein_id(GenBank) gb|AAG03871.1|AE004485_12|gnl|PseudoCAP|PA0482
TIGR NTL03PA00483
UniParc UPI00000C507F
UniProtKB Acc Q9I636
UniProtKB ID MASZ_PSEAE
UniRef100 UniRef100_Q9I636
UniRef50 UniRef50_P37330
UniRef90 UniRef90_Q9I636

Product

Feature Type CDS
Coding Frame 1
Product Name
malate synthase G
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.19
Kyte-Doolittle Hydrophobicity Value -0.251
Molecular Weight (kDa) 78.7
Isoelectric Point (pI) 5.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VFB TRANSFERASE 04/07/17 1.36 Angstrom Resolution Crystal Structure of Malate Synthase G from Pseudomonas aeruginosa in Complex with Glycolic Acid. PSEUDOMONAS AERUGINOSA (STRAIN PAO1) 1.36 X-RAY DIFFRACTION 100.0
5OAS TRANSFERASE 06/23/17 Crystal structure of malate synthase G from Pseudomonas aeruginosa in apo form. PSEUDOMONAS AERUGINOSA 1.62 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 142 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000471 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0482
Search term: glcB
Search term: malate synthase G

References

Molecular characterization of Escherichia coli malate synthase G. Differentiation with the malate synthase A isoenzyme.
Molina I, Pellicer MT, Badia J, Aguilar J, Baldoma L
Eur. J. Biochem. 1994 Sep;224(2):541-8
PubMed ID: 7925370
Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme.
Reinscheid DJ, Eikmanns BJ, Sahm H
Microbiology (Reading, Engl.) 1994 Nov;140 ( Pt 11)
PubMed ID: 7812449
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Biochemical characterization of malate synthase G of P. aeruginosa.
Roucourt B, Minnebo N, Augustijns P, Hertveldt K, Volckaert G, Lavigne R
BMC Biochem. 2009 Jun;10
PubMed ID: 19549344