Pseudomonas aeruginosa PAO1, PA0500 (bioB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0500
Name
bioB
Replicon chromosome
Genomic location 559644 - 560702 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249191.1
GI 15595697
Affymetrix PA0500_bioB_at
DNASU PaCD00006210
Entrez 877668
GenBank AAG03889.1
INSDC AAG03889.1
NCBI Locus Tag PA0500
protein_id(GenBank) gb|AAG03889.1|AE004487_3|gnl|PseudoCAP|PA0500
TIGR NTL03PA00501
UniParc UPI00000C5090
UniProtKB Acc Q9I618
UniProtKB ID BIOB_PSEAE
UniRef100 UniRef100_B7V485
UniRef50 UniRef50_A9EXH2
UniRef90 UniRef90_A4XZR9

Product

Feature Type CDS
Coding Frame 1
Product Name
biotin synthase
Product Name Confidence: Class 2
Synonyms biotin synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.77
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 39.1
Isoelectric Point (pI) 6.01

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 475 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000487 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0500
Search term: bioB
Search term: biotin synthase

References

Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product.
Sanyal I, Cohen G, Flint DH
Biochemistry 1994 Mar;33(12):3625-31
PubMed ID: 8142361
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use.
Shaw NM, Birch OM, Tinschert A, Venetz V, Dietrich R, Savoy LA
Biochem. J. 1998 Mar;330 ( Pt 3)
PubMed ID: 9494071
Transfer of sulfur to biotin from biotin synthase (BioB protein).
Gibson KJ, Pelletier DA, Turner IM
Biochem. Biophys. Res. Commun. 1999 Jan;254(3):632-5
PubMed ID: 9920791