Pseudomonas aeruginosa PAO1, PA0504 (bioD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0504
Name
bioD
Replicon chromosome
Genomic location 563549 - 564235 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249195.1
GI 15595701
Affymetrix PA0504_bioD_at
DNASU PaCD00006212
Entrez 878220
GenBank AAG03893.1
INSDC AAG03893.1
NCBI Locus Tag PA0504
protein_id(GenBank) gb|AAG03893.1|AE004487_7|gnl|PseudoCAP|PA0504
TIGR NTL03PA00505
UniParc UPI000012697C
UniProtKB Acc Q9I614
UniProtKB ID BIOD_PSEAE
UniRef100 UniRef100_Q9I614
UniRef50 UniRef50_Q9I614
UniRef90 UniRef90_Q9I614

Product

Feature Type CDS
Coding Frame 1
Product Name
dethiobiotin synthase
Product Name Confidence: Class 2
Synonyms DTB synthetase
DTBS
dethiobiotin synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.92
Kyte-Doolittle Hydrophobicity Value 0.501
Molecular Weight (kDa) 23.3
Isoelectric Point (pI) 5.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 405 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000491 (555 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0504
Search term: bioD

References

The purification and properties of dethiobiotin synthetase.
Krell K, Eisenberg MA
J Biol Chem 1970 Dec 25;245(24):6558-66
PubMed ID: 4921568
Sequence and crystallization of Escherichia coli dethiobiotin synthetase, the penultimate enzyme of biotin biosynthesis.
Alexeev D, Bury SM, Boys CW, Turner MA, Sawyer L, Ramsey AJ, Baxter HC, Baxter RL
J. Mol. Biol. 1994 Jan;235(2):774-6
PubMed ID: 8289297
Crystal structure of an ATP-dependent carboxylase, dethiobiotin synthetase, at 1.65 A resolution.
Huang W, Lindqvist Y, Schneider G, Gibson KJ, Flint D, Lorimer G
Structure 1994 May;2(5):407-14
PubMed ID: 8081756
Active site mutants of Escherichia coli dethiobiotin synthetase: effects of mutations on enzyme catalytic and structural properties.
Yang G, Sandalova T, Lohman K, Lindqvist Y, Rendina AR
Biochemistry 1997 Apr;36(16):4751-60
PubMed ID: 9125495