Pseudomonas aeruginosa PAO1, PA0575

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0575
Name
Replicon chromosome
Genomic location 630527 - 634264 (- strand)
Transposon Mutants 9 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249266.1
GI 15595772
Affymetrix PA0575_at
Entrez 882126
GenBank AAG03964.1
INSDC AAG03964.1
NCBI Locus Tag PA0575
protein_id(GenBank) gb|AAG03964.1|AE004493_9|gnl|PseudoCAP|PA0575
TIGR NTL03PA00576
UniParc UPI00000C50C3
UniProtKB Acc Q9I5W1
UniProtKB ID Q9I5W1_PSEAE
UniRef100 UniRef100_Q9I5W1
UniRef50 UniRef50_Q4K4W0
UniRef90 UniRef90_Q9I5W1

Product

Feature Type CDS
Coding Frame 1
Product Name
conserved hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -30.54
Kyte-Doolittle Hydrophobicity Value -0.178
Molecular Weight (kDa) 139.7
Isoelectric Point (pI) 5.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5M3C HYDROLASE 10/14/16 Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain PSEUDOMONAS AERUGINOSA 2.8 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 564 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000558 (508 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0575

References

PAS domains: internal sensors of oxygen, redox potential, and light.
Taylor BL, Zhulin IB
Microbiol. Mol. Biol. Rev. 1999 Jun;63(2):479-506
PubMed ID: 10357859
Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.
Mantoni F, Paiardini A, Brunotti P, D'Angelo C, Cervoni L, Paone A, Cappellacci L, Petrelli R, Ricciutelli M, Leoni L, Rampioni G, Arcovito A, Rinaldo S, Cutruzzolà F, Giardina G.
FEBS J. 2018 Oct;285(20):3815-3834
PubMed ID: 30106221