Pseudomonas aeruginosa PAO1, PA0576 (rpoD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0576
Name
rpoD
Synonym: rpoDA
Replicon chromosome
Genomic location 634371 - 636224 (- strand)

Cross-References

RefSeq NP_249267.1
GI 15595773
Affymetrix PA0576_rpoD_at
Entrez 882038
GenBank AAG03965.1
INSDC AAG03965.1
NCBI Locus Tag PA0576
protein_id(GenBank) gb|AAG03965.1|AE004494_1|gnl|PseudoCAP|PA0576
TIGR NTL03PA00577
UniParc UPI0000134834
UniProtKB Acc P26480
UniProtKB ID RPOD_PSEAE
UniRef100 UniRef100_P26480
UniRef50 UniRef50_P26480
UniRef90 UniRef90_P26480

Product

Feature Type CDS
Coding Frame 1
Product Name
sigma factor RpoD
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (69.64 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -31.01
Kyte-Doolittle Hydrophobicity Value -0.593
Molecular Weight (kDa) 69.6
Isoelectric Point (pI) 4.62

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000559 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0576
Search term: rpoD
Search term: sigma factor RpoD

References

Cloning and analysis of the gene (rpoDA) for the principal sigma factor of Pseudomonas aeruginosa.
Tanaka K, Takahashi H
Biochim Biophys Acta 1991 May 2;1089(1):113-9
PubMed ID: 1902749
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344