Pseudomonas aeruginosa PAO1, PA0577 (dnaG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0577
Name
dnaG
Replicon chromosome
Genomic location 636304 - 638298 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249268.1
GI 15595774
Affymetrix PA0577_dnaG_at
Entrez 882047
GenBank AAG03966.1
INSDC AAG03966.1
NCBI Locus Tag PA0577
protein_id(GenBank) gb|AAG03966.1|AE004494_2|gnl|PseudoCAP|PA0577
TIGR NTL03PA00578
UniParc UPI00001321E8
UniProtKB Acc Q9I5W0
UniProtKB ID DNAG_PSEAE
UniRef100 UniRef100_Q9I5W0
UniRef50 UniRef50_Q9I5W0
UniRef90 UniRef90_Q9I5W0

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA primase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.74
Kyte-Doolittle Hydrophobicity Value -0.548
Molecular Weight (kDa) 74.2
Isoelectric Point (pI) 7.27

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VAZ TRANSFERASE 03/28/17 Crystal structure of a DNA primase domain from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.4 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000560 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0577
Search term: dnaG
Search term: DNA primase

References

The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
Burton ZF, Gross CA, Watanabe KK, Burgess RR
Cell 1983 Feb;32(2):335-49
PubMed ID: 6186393
Enriched sources of Escherichia coli replication proteins. The dnaG primase is a zinc metalloprotein.
Stamford NP, Lilley PE, Dixon NE
Biochim Biophys Acta 1992 Aug 17;1132(1):17-25
PubMed ID: 1511009
Cloning and analysis of the dnaG gene encoding Pseudomonas putida DNA primase.
Szafranski P, Smith CL, Cantor CR
Biochim. Biophys. Acta 1997 Jun;1352(3):243-8
PubMed ID: 9224947