Pseudomonas aeruginosa PAO1, PA0620

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0620
Name
Replicon chromosome
Genomic location 678821 - 680896 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249311.1
GI 15595817
Affymetrix PA0620_at
Entrez 877993
GenBank AAG04009.1
INSDC AAG04009.1
NCBI Locus Tag PA0620
protein_id(GenBank) gb|AAG04009.1|AE004498_4|gnl|PseudoCAP|PA0620
TIGR NTL03PA00621
UniParc UPI00000D4281
UniProtKB Acc G3XD71
UniProtKB ID G3XD71_PSEAE
UniRef100 UniRef100_Q9S576
UniRef50 UniRef50_Q9S576
UniRef90 UniRef90_Q9S576

Product

Feature Type CDS
Coding Frame 1
Product Name
probable bacteriophage protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected using LC-MS/MS (72.29 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.01
Kyte-Doolittle Hydrophobicity Value -0.120
Molecular Weight (kDa) 72.3
Isoelectric Point (pI) 8.39

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Extracellular Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6CT8 VIRAL PROTEIN 03/22/18 Selenomethionine structure of N-truncated R2-type pyocin tail fiber at 2.6 angstrom resolution PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.62 X-RAY DIFFRACTION 100.0
6CL6 VIRAL PROTEIN 03/01/18 Structure of P. aeruginosa R2 pyocin fiber PA0620 comprising C-terminal residues 323-691 PSEUDOMONAS AERUGINOSA 1.898 X-RAY DIFFRACTION 99.7
6CU2 VIRAL PROTEIN 03/23/18 Structure of N-truncated R2-type pyocin tail fiber at 2.6 angstrom resolution PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.58 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 55 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000603 (342 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0620

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages.
Haggård-Ljungquist E, Halling C, Calendar R
J Bacteriol 1992 Mar;174(5):1462-77
PubMed ID: 1531648
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344