Pseudomonas aeruginosa PAO1, PA0672 (hemO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0672
Name
hemO
Synonym: pigA
Replicon chromosome
Genomic location 732959 - 733555 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249363.1
GI 15595869
Affymetrix PA0672_at
Entrez 880820
GenBank AAG04061.1
INSDC AAG04061.1
NCBI Locus Tag PA0672
protein_id(GenBank) gb|AAG04061.1|AE004502_4|gnl|PseudoCAP|PA0672
TIGR NTL03PA00673
UniParc UPI00000D4231
UniProtKB Acc G3XCZ8
UniProtKB ID G3XCZ8_PSEAE
UniRef100 UniRef100_G3XCZ8
UniRef50 UniRef50_Q4K7S1
UniRef90 UniRef90_G3XCZ8

Product

Feature Type CDS
Coding Frame 1
Product Name
heme oxygenase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.0, molecular weight:21.9, PMID:21674802)
Charge (pH 7) -8.03
Kyte-Doolittle Hydrophobicity Value -0.418
Molecular Weight (kDa) 22.0
Isoelectric Point (pI) 4.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1SK7 OXIDOREDUCTASE 03/04/04 Structural Basis for Novel Delta-Regioselective Heme Oxygenation in the Opportunistic Pathogen Pseudomonas aeruginosa Pseudomonas aeruginosa 1.6 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 23 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000651 (473 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0672
Search term: hemO
Search term: heme oxygenase

Human Homologs

References

Degradation of heme in gram-negative bacteria: the product of the hemO gene of Neisseriae is a heme oxygenase.
Zhu W, Wilks A, Stojiljkovic I
J Bacteriol 2000 Dec;182(23):6783-90
PubMed ID: 11073924
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
The cytoplasmic heme-binding protein (PhuS) from the heme uptake system of Pseudomonas aeruginosa is an intracellular heme-trafficking protein to the delta-regioselective heme oxygenase.
Lansky IB, Lukat-Rodgers GS, Block D, Rodgers KR, Ratliff M, Wilks A
J Biol Chem 2006 May 12;281(19):13652-13662
PubMed ID: 16533806