Pseudomonas aeruginosa PAO1, PA0745 (dspI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0745
Name
dspI
Synonym: dispersion inducer
Replicon chromosome
Genomic location 812445 - 813263 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_249436.1
GI 15595942
Affymetrix PA0745_at
Entrez 878156
GenBank AAG04134.1
INSDC AAG04134.1
NCBI Locus Tag PA0745
protein_id(GenBank) gb|AAG04134.1|AE004509_9|gnl|PseudoCAP|PA0745
TIGR NTL03PA00746
UniParc UPI00000C5134
UniProtKB Acc Q9I5I4
UniProtKB ID Q9I5I4_PSEAE
UniRef100 UniRef100_W0YNS2
UniRef50 UniRef50_A9ANK2
UniRef90 UniRef90_W6RIF3

Product

Feature Type CDS
Coding Frame 1
Product Name
DspI
Product Name Confidence: Class 1
Synonyms probable enoyl-CoA hydratase/isomerase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.94
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 29.9
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WYB ISOMERASE 01/12/17 Structure of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PA14 2.25 X-RAY DIFFRACTION 99.6
5WYD ISOMERASE 01/12/17 Structural of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PAO1 2.101 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 508 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000719 (1195 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0745
Search term: dspI
Search term: DspI

Human Homologs

Ensembl 97, assembly GRCh38.p12
enoyl-CoA hydratase, short chain 1 [Source:HGNC Symbol;Acc:HGNC:3151]
E-value: 1.8e-28
Percent Identity: 33.6

References

Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis.
Liu L, Li T, Cheng XJ, Peng CT, Li CC, He LH, Ju SM, Wang NY, Ye TH, Lian M, Xiao QJ, Song YJ, Zhu YB, Yu LT, Wang ZL, Bao R
Sci Rep 2018 Mar 2;8(1):3928
PubMed ID: 29500457
Genome-wide identification of Pseudomonas aeruginosa virulence-related genes using a Caenorhabditis elegans infection model.
Feinbaum RL, Urbach JM, Liberati NT, Djonovic S, Adonizio A, Carvunis AR, Ausubel FM
PLoS Pathog. 2012 ;8(7):e1002813
PubMed ID: 22911607