Pseudomonas aeruginosa PAO1, PA0755 (opdH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0755
Name
opdH
Synonym: opdH
Replicon chromosome
Genomic location 822915 - 824198 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249446.1
GI 15595952
Affymetrix PA0755_at
Entrez 882059
GenBank AAG04144.1
INSDC AAG04144.1
NCBI Locus Tag PA0755
protein_id(GenBank) gb|AAG04144.1|AE004510_8|gnl|PseudoCAP|PA0755
TIGR NTL03PA00756
UniParc UPI00000C513D
UniProtKB Acc Q9I5H4
UniProtKB ID Q9I5H4_PSEAE
UniRef100 UniRef100_Q9I5H4
UniRef50 UniRef50_W6QYC2
UniRef90 UniRef90_Q9I5H4

Product

Feature Type CDS
Coding Frame 1
Product Name
cis-aconitate porin OpdH
Product Name Confidence: Class 1
Synonyms OccK5
cis-aconitate porin OpdH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.21
Kyte-Doolittle Hydrophobicity Value -0.448
Molecular Weight (kDa) 47.0
Isoelectric Point (pI) 8.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3T20 MEMBRANE PROTEIN 07/22/11 Crystal structure of Pseudomonas aeruginosa OccK5 (OpdH) Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0
2Y0L TRANSPORT PROTEIN 12/03/10 Crystal Structure of Pseudomonas Aeruginosa OpdO PSEUDOMONAS AERUGINOSA 2.59 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 21 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000729 (839 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0755
Search term: opdH

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Responses of carbapenemase-producing and non-producing carbapenem-resistant Pseudomonas aeruginosa strains to meropenem revealed by quantitative tandem mass spectrometry proteomics.
Salvà-Serra F, Jaén-Luchoro D, Marathe NP, Adlerberth I, Moore ERB, Karlsson R
Front Microbiol 2022;13:1089140
PubMed ID: 36845973
Role of the novel OprD family of porins in nutrient uptake in Pseudomonas aeruginosa.
Tamber S, Ochs MM, Hancock RE
J Bacteriol 2006 Jan;188(1):45-54
PubMed ID: 16352820
OccK channels from Pseudomonas aeruginosa exhibit diverse single-channel electrical signatures but conserved anion selectivity.
Liu J, Eren E, Vijayaraghavan J, Cheneke BR, Indic M, van den Berg B, Movileanu L
Biochemistry 2012 Mar 20;51(11):2319-30
PubMed ID: 22369314