Pseudomonas aeruginosa PAO1, PA0762 (algU)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0762
Name
algU
Synonym: algT
Replicon chromosome
Genomic location 831301 - 831882 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249453.1
GI 15595959
Affymetrix PA0762_algU_at
DNASU PaCD00006350
Entrez 882125
GenBank AAG04151.1
INSDC AAA92355.1
NCBI Locus Tag PA0762
protein_id(GenBank) gb|AAG04151.1|AE004511_4|gnl|PseudoCAP|PA0762
TIGR NTL03PA00763
UniParc UPI000013484A
UniProtKB Acc Q06198
UniProtKB ID RPSH_PSEAE
UniRef100 UniRef100_Q06198
UniRef50 UniRef50_D0ZSY9
UniRef90 UniRef90_Q06198

Product

Feature Type CDS
Coding Frame 1
Product Name
sigma factor AlgU
Product Name Confidence: Class 1
Synonyms sigma factor AlgT
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.83
Kyte-Doolittle Hydrophobicity Value -0.428
Molecular Weight (kDa) 22.2
Isoelectric Point (pI) 5.38

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate regulation
Evidence
Host Organism
Notes
Reference
Source 2
Database Victors : 4262
Category Alginate regulation
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 495 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000736 (549 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0762
Search term: algU
Search term: sigma factor AlgU

References

Functional equivalence of Escherichia coli sigma E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa.
Yu H, Schurr MJ, Deretic V
J. Bacteriol. 1995 Jun;177(11):3259-68
PubMed ID: 7768826
Control of AlgU, a member of the sigma E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis.
Schurr MJ, Yu H, Martinez-Salazar JM, Boucher JC, Deretic V
J. Bacteriol. 1996 Aug;178(16):4997-5004
PubMed ID: 8759866
A complex multilevel attack on Pseudomonas aeruginosa algT/U expression and algT/U activity results in the loss of alginate production.
Sautter R, Ramos D, Schneper L, Ciofu O, Wassermann T, Koh CL, Heydorn A, Hentzer M, Høiby N, Kharazmi A, Molin S, Devries CA, Ohman DE, Mathee K
Gene 2012 May;498(2):242-53
PubMed ID: 22088575