Pseudomonas aeruginosa PAO1, PA0763 (mucA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0763
Name
mucA
Replicon chromosome
Genomic location 831914 - 832498 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_249454.1
GI 15595960
Affymetrix PA0763_mucA_at
Entrez 879357
GenBank AAG04152.1
INSDC AAG04152.1
NCBI Locus Tag PA0763
protein_id(GenBank) gb|AAG04152.1|AE004511_5|gnl|PseudoCAP|PA0763
TIGR NTL03PA00764
UniParc UPI000012F8D3
UniProtKB Acc P38107
UniProtKB ID MUCA_PSEAE
UniRef100 UniRef100_P38107
UniRef50 UniRef50_P38107
UniRef90 UniRef90_P38107

Product

Feature Type CDS
Coding Frame 1
Product Name
anti-sigma factor MucA
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.31
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 5.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IN7 TRANSCRIPTION 10/24/18 Crystal structure of AlgU in complex with MucA(cyto) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.96 X-RAY DIFFRACTION 100.0
6IN9 MEMBRANE PROTEIN 10/24/18 Crystal structure of MucB in complex with MucA(peri) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.801 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database Victors : 4227
Category Alginate regulation
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Medicago sativa
Notes mucA mutant attenuated for virulence in alfalfa seedling Infection model
Reference 12426404
Source 2
Database VFDB : VF0091
Category Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000737 (510 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0763
Search term: mucA

References

Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN).
Mathee K, McPherson CJ, Ohman DE
J Bacteriol 1997 Jun;179(11):3711-20
PubMed ID: 9171421
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients.
Martin DW, Schurr MJ, Mudd MH, Govan JR, Holloway BW, Deretic V
Proc. Natl. Acad. Sci. U.S.A. 1993 Sep;90(18):8377-81
PubMed ID: 8378309
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J. Bacteriol. 2009 Apr;191(7):2285-95
PubMed ID: 19168621
Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy.
Schurr MJ, Martin DW, Mudd MH, Deretic V
J. Bacteriol. 1994 Jun;176(11):3375-82
PubMed ID: 8195094
Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA.
Xie ZD, Hershberger CD, Shankar S, Ye RW, Chakrabarty AM
J. Bacteriol. 1996 Aug;178(16):4990-6
PubMed ID: 8759865
Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control.
Fyfe JA, Govan JR
J. Gen. Microbiol. 1980 Aug;119(2):443-50
PubMed ID: 6785378
Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate.
Ohman DE, Chakrabarty AM
Infect. Immun. 1981 Jul;33(1):142-8
PubMed ID: 6790439