Pseudomonas aeruginosa PAO1, PA0764 (mucB)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: algN
Replicon chromosome
Genomic location 832507 - 833457 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq NP_249455.1
GI 15595961
Affymetrix PA0764_mucB_at
Entrez 882064
GenBank AAG04153.1
NCBI Locus Tag PA0764
protein_id(GenBank) gb|AAG04153.1|AE004511_6|gnl|PseudoCAP|PA0764
UniProtKB Acc P38108


Feature Type CDS
Coding Frame 1
Product Name
negative regulator for alginate biosynthesis MucB
Product Name Confidence: Class 1
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.88
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 34.6
Isoelectric Point (pI) 6.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate regulation
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 110 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000738 (544 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0764
Search term: mucB


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species.
Goldberg JB, Gorman WL, Flynn JL, Ohman DE
J. Bacteriol. 1993 Mar;175(5):1303-8
PubMed ID: 8444793
Differentiation of Pseudomonas aeruginosa into the alginate-producing form: inactivation of mucB causes conversion to mucoidy.
Martin DW, Schurr MJ, Mudd MH, Deretic V
Mol. Microbiol. 1993 Aug;9(3):497-506
PubMed ID: 8412698
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J. Bacteriol. 2011 Jan;193(1):286-91
PubMed ID: 21036998
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J. Bacteriol. 2009 Apr;191(7):2285-95
PubMed ID: 19168621
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994