Pseudomonas aeruginosa PAO1, PA0764 (mucB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0764
Name
mucB
Synonym: algN
Replicon chromosome
Genomic location 832507 - 833457 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249455.1
GI 15595961
Affymetrix PA0764_mucB_at
Entrez 882064
GenBank AAG04153.1
NCBI Locus Tag PA0764
protein_id(GenBank) gb|AAG04153.1|AE004511_6|gnl|PseudoCAP|PA0764
TIGR NTL03PA00765
UniProtKB Acc P38108
UniProtKB ID MUCB_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
negative regulator for alginate biosynthesis MucB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.88
Kyte-Doolittle Hydrophobicity Value -0.238
Molecular Weight (kDa) 34.6
Isoelectric Point (pI) 6.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0091
Category Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 110 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000738 (544 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0764
Search term: mucB

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species.
Goldberg JB, Gorman WL, Flynn JL, Ohman DE
J. Bacteriol. 1993 Mar;175(5):1303-8
PubMed ID: 8444793
Differentiation of Pseudomonas aeruginosa into the alginate-producing form: inactivation of mucB causes conversion to mucoidy.
Martin DW, Schurr MJ, Mudd MH, Deretic V
Mol. Microbiol. 1993 Aug;9(3):497-506
PubMed ID: 8412698
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J. Bacteriol. 2011 Jan;193(1):286-91
PubMed ID: 21036998
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J. Bacteriol. 2009 Apr;191(7):2285-95
PubMed ID: 19168621
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994