Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0764
|
Name |
mucB
Synonym: algN |
Replicon | chromosome |
Genomic location | 832507 - 833457 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 2 transposon mutants in orthologs |
RefSeq | NP_249455.1 |
GI | 15595961 |
Affymetrix | PA0764_mucB_at |
Entrez | 882064 |
GenBank | AAG04153.1 |
NCBI Locus Tag | PA0764 |
protein_id(GenBank) | gb|AAG04153.1|AE004511_6|gnl|PseudoCAP|PA0764 |
TIGR | NTL03PA00765 |
UniProtKB Acc | P38108 |
UniProtKB ID | MUCB_PSEAE |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
negative regulator for alginate biosynthesis MucB
|
Synonyms | |
Evidence for Translation |
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | -2.88 |
Kyte-Doolittle Hydrophobicity Value | -0.238 |
Molecular Weight (kDa) | 34.6 |
Isoelectric Point (pI) | 6.06 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
6IN9 | MEMBRANE PROTEIN | 10/24/18 | Crystal structure of MucB in complex with MucA(peri) | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 1.801 | X-RAY DIFFRACTION | 100.0 |
6JAU | PROTEIN BINDING | 01/25/19 | The complex structure of Pseudomonas aeruginosa MucA/MucB. | Pseudomonas aeruginosa (strain PAO1) | 1.905 | X-RAY DIFFRACTION | 100.0 |
6IN8 | MEMBRANE PROTEIN | 10/24/18 | Crystal structure of MucB | Pseudomonas aeruginosa | 2.2 | X-RAY DIFFRACTION | 99.7 |
Source 1 | |
Database | VFDB : VF0091 |
Category | Alginate regulation |
Evidence |
|
Host Organism | |
Notes | |
Reference |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 110 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000738 (527 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0764
Search term: mucB
Search term: negative regulator for alginate biosynthesis MucB
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species.
Goldberg JB, Gorman WL, Flynn JL, Ohman DE
J Bacteriol 1993 Mar;175(5):1303-8
PubMed ID: 8444793
|
Differentiation of Pseudomonas aeruginosa into the alginate-producing form: inactivation of mucB causes conversion to mucoidy.
Martin DW, Schurr MJ, Mudd MH, Deretic V
Mol Microbiol 1993 Aug;9(3):497-506
PubMed ID: 8412698
|
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J Bacteriol 2011 Jan;193(1):286-91
PubMed ID: 21036998
|
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc Natl Acad Sci U S A 2007 May 8;104(19):8107-12
PubMed ID: 17470813
|
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J Bacteriol 2009 Apr;191(7):2285-95
PubMed ID: 19168621
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|