Pseudomonas aeruginosa PAO1, PA0766 (mucD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0766
Name
mucD
Replicon chromosome
Genomic location 833949 - 835373 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249457.1
GI 15595963
Affymetrix PA0766_mucD_at
Entrez 879207
GenBank AAG04155.1
INSDC AAG04155.1
NCBI Locus Tag PA0766
protein_id(GenBank) gb|AAG04155.1|AE004511_8|gnl|PseudoCAP|PA0766
TIGR NTL03PA00767
UniParc UPI00000D4255
UniProtKB Acc G3XD20
UniProtKB ID G3XD20_PSEAE
UniRef100 UniRef100_W0YL26
UniRef50 UniRef50_A4XSC0
UniRef90 UniRef90_A6VAL0

Product

Feature Type CDS
Coding Frame 1
Product Name
serine protease MucD precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (50.32 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.43
Kyte-Doolittle Hydrophobicity Value -0.057
Molecular Weight (kDa) 50.3
Isoelectric Point (pI) 7.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization
Detected in a periplasmic protein preparation by spheroplasting by lysozyme and sucrose.(PMID:19333994)

Virulence Evidence

Source 1
Database VFDB
Category Alginate regulation
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 616 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000740 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0766
Search term: mucD

Human Homologs

Ensembl 94, assembly GRCh38.p12
HtrA serine peptidase 1 [Source:HGNC Symbol;Acc:HGNC:9476]
E-value: 8.4e-42
Percent Identity: 35.4

References

Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA.
Boucher JC, Martinez-Salazar J, Schurr MJ, Mudd MH, Yu H, Deretic V
J. Bacteriol. 1996 Jan;178(2):511-23
PubMed ID: 8550474
Pseudomonas aeruginosa MucD regulates the alginate pathway through activation of MucA degradation via MucP proteolytic activity.
Damron FH, Yu HD
J. Bacteriol. 2011 Jan;193(1):286-91
PubMed ID: 21036998
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344